Detection of Legionella pneumophila in biofilms containing a complex microbial consortium by gas chromatography-mass spectrometry analysis of genus-specific hydroxy fatty acids
Detection of Legionella pneumophila in biofilms containing a complex microbial consortium by gas chromatography-mass spectrometry analysis of genus-specific hydroxy fatty acids
A gas chromatographic-mass spectrometric method was used to detect Legionella pneumophila in biofilms in potable water containing a complex microbial consortium. The unique 3-hydroxy and 2,3-dihydroxy fatty acids of the L. pneumophila lipopolysaccharides (LPS) were detected in both the planktonic phase of the continuous culture model and in the biofilms forming on both copper and polyethylene substrata. The technique confirmed that lower numbers of Legionella colonised and grew on copper in comparison to polyethylene and offers promise for routine detection of Legionella in biofilms in the environment.
2,3-Dihydroxy fatty acids, 3-Hydroxy fatty acids, Biofilms, Continuous culture, Legionella, Lipopolysaccharide
139-144
Walker, James T.
2bb5ed4e-d929-47e4-97ba-70641716acd7
Sonesson, Anders
698d5117-7a01-44a6-ae65-d9bc6c12be58
William Keevil, C.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
White, David C.
d5fee82a-2275-4478-82b3-04f0b39f5111
15 October 1993
Walker, James T.
2bb5ed4e-d929-47e4-97ba-70641716acd7
Sonesson, Anders
698d5117-7a01-44a6-ae65-d9bc6c12be58
William Keevil, C.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
White, David C.
d5fee82a-2275-4478-82b3-04f0b39f5111
Walker, James T., Sonesson, Anders, William Keevil, C. and White, David C.
(1993)
Detection of Legionella pneumophila in biofilms containing a complex microbial consortium by gas chromatography-mass spectrometry analysis of genus-specific hydroxy fatty acids.
FEMS Microbiology Letters, 113 (2), .
(doi:10.1111/j.1574-6968.1993.tb06504.x).
Abstract
A gas chromatographic-mass spectrometric method was used to detect Legionella pneumophila in biofilms in potable water containing a complex microbial consortium. The unique 3-hydroxy and 2,3-dihydroxy fatty acids of the L. pneumophila lipopolysaccharides (LPS) were detected in both the planktonic phase of the continuous culture model and in the biofilms forming on both copper and polyethylene substrata. The technique confirmed that lower numbers of Legionella colonised and grew on copper in comparison to polyethylene and offers promise for routine detection of Legionella in biofilms in the environment.
This record has no associated files available for download.
More information
Published date: 15 October 1993
Additional Information:
© 1993 Federation of European Microbiological Societies
Keywords:
2,3-Dihydroxy fatty acids, 3-Hydroxy fatty acids, Biofilms, Continuous culture, Legionella, Lipopolysaccharide
Identifiers
Local EPrints ID: 468199
URI: http://eprints.soton.ac.uk/id/eprint/468199
ISSN: 0378-1097
PURE UUID: 1f2f61ce-7e7c-471c-bcf8-83313a2ab27f
Catalogue record
Date deposited: 04 Aug 2022 16:58
Last modified: 17 Mar 2024 02:54
Export record
Altmetrics
Contributors
Author:
James T. Walker
Author:
Anders Sonesson
Author:
David C. White
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
