The proteins of human lung surfactant
The proteins of human lung surfactant
Human pulmonary surfactant was purified from bronchoalveolar lavage of patients. The proteins present in surfactant were analyzed by SDS-polyacrylamide gel electrophoresis into serum and non-serum components. One non-serum surfactant protein (Mr = 43 000) was then identified in the 100 000 X g supernatant of a lung homogenate on the basis of phospholipid binding. This lung protein was purified and partially characterized. The presence of 3-methyl histidine and reaction in Western blot analysis with antibody against chicken muscle actin both strongly suggested that the 43 000 Da protein of human surfactant is indeed cytoplasmic actin. It is proposed that this surfactant protein is involved in the secretion and not necessarily in the function of surfactant.
303-313
Hunt, A.N.
95a3e223-da96-40e7-b47d-27dce014e305
Normand, I.C.S.
93c0816b-c5a0-4abf-b4c9-b4cf377aae48
Postle, A.D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
4 December 1985
Hunt, A.N.
95a3e223-da96-40e7-b47d-27dce014e305
Normand, I.C.S.
93c0816b-c5a0-4abf-b4c9-b4cf377aae48
Postle, A.D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Hunt, A.N., Normand, I.C.S. and Postle, A.D.
(1985)
The proteins of human lung surfactant.
Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, 837 (3), .
Abstract
Human pulmonary surfactant was purified from bronchoalveolar lavage of patients. The proteins present in surfactant were analyzed by SDS-polyacrylamide gel electrophoresis into serum and non-serum components. One non-serum surfactant protein (Mr = 43 000) was then identified in the 100 000 X g supernatant of a lung homogenate on the basis of phospholipid binding. This lung protein was purified and partially characterized. The presence of 3-methyl histidine and reaction in Western blot analysis with antibody against chicken muscle actin both strongly suggested that the 43 000 Da protein of human surfactant is indeed cytoplasmic actin. It is proposed that this surfactant protein is involved in the secretion and not necessarily in the function of surfactant.
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Published date: 4 December 1985
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Local EPrints ID: 468569
URI: http://eprints.soton.ac.uk/id/eprint/468569
PURE UUID: 32598067-e15e-4c99-955a-46ccac9d9739
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Date deposited: 18 Aug 2022 16:39
Last modified: 19 Aug 2022 01:33
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Author:
A.N. Hunt
Author:
I.C.S. Normand
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