Sequence of a small subunit rRNA gene of Schistosoma mansoni and its use in phylogenetic analysis
Sequence of a small subunit rRNA gene of Schistosoma mansoni and its use in phylogenetic analysis
The complete sequence of a small ribosomal RNA gene of Schistosoma mansoni contained within plasmids pSM389 and pSM889 is presented. It was found to be 1992 bp in length, larger than that of most eukaryotes. Extra nucleotides occur in regions known to be variable (V4 and V7). The predicted secondary structure of the nucleotides 660-853 revealed additional helices which have been designated E21-1A and E21-1B. The other region to differ from higher eukaryotes lies between nucleotides 1457 and 1569. Secondary structure prediction demonstrated that a single extended helix may be formed from this part of the schistosome small subunit rRNA sequence. Nucleotides that could be unambiguously aligned with those of 12 other eukaryotes were used to estimate phylogenetic relationships. The consensus tree obtained by Maximum Parsimony analysis showed the schistosome as a sister taxon to the flatworm Dugesia tigrina.
Amino Acid Sequence, Animals, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, RNA, Ribosomal/chemistry, Schistosoma mansoni/genetics
201-8
Ali, P O
29e8cc90-f534-4013-acf2-f3f9400498fb
Simpson, A J
2415f675-edef-4059-8678-8f0995ba9521
Allen, R
19be5230-fb52-48db-b720-d91ab39718b9
Waters, A P
ccfe468e-33ee-4b5a-8468-191ea455bf35
Humphries, C J
4c84bc90-1506-4351-a852-d0078feb48a9
Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Rollinson, D
917d0e09-176f-4058-89ff-ec2ad3a496ad
June 1991
Ali, P O
29e8cc90-f534-4013-acf2-f3f9400498fb
Simpson, A J
2415f675-edef-4059-8678-8f0995ba9521
Allen, R
19be5230-fb52-48db-b720-d91ab39718b9
Waters, A P
ccfe468e-33ee-4b5a-8468-191ea455bf35
Humphries, C J
4c84bc90-1506-4351-a852-d0078feb48a9
Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Rollinson, D
917d0e09-176f-4058-89ff-ec2ad3a496ad
Ali, P O, Simpson, A J, Allen, R, Waters, A P, Humphries, C J, Johnston, D A and Rollinson, D
(1991)
Sequence of a small subunit rRNA gene of Schistosoma mansoni and its use in phylogenetic analysis.
Molecular and Biochemical Parasitology, 46 (2), .
(doi:10.1016/0166-6851(91)90044-7).
Abstract
The complete sequence of a small ribosomal RNA gene of Schistosoma mansoni contained within plasmids pSM389 and pSM889 is presented. It was found to be 1992 bp in length, larger than that of most eukaryotes. Extra nucleotides occur in regions known to be variable (V4 and V7). The predicted secondary structure of the nucleotides 660-853 revealed additional helices which have been designated E21-1A and E21-1B. The other region to differ from higher eukaryotes lies between nucleotides 1457 and 1569. Secondary structure prediction demonstrated that a single extended helix may be formed from this part of the schistosome small subunit rRNA sequence. Nucleotides that could be unambiguously aligned with those of 12 other eukaryotes were used to estimate phylogenetic relationships. The consensus tree obtained by Maximum Parsimony analysis showed the schistosome as a sister taxon to the flatworm Dugesia tigrina.
This record has no associated files available for download.
More information
Published date: June 1991
Keywords:
Amino Acid Sequence, Animals, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, RNA, Ribosomal/chemistry, Schistosoma mansoni/genetics
Identifiers
Local EPrints ID: 468595
URI: http://eprints.soton.ac.uk/id/eprint/468595
ISSN: 0166-6851
PURE UUID: 53a1ba71-be94-4da7-ab69-dc1cc7c8e3f0
Catalogue record
Date deposited: 18 Aug 2022 16:42
Last modified: 17 Mar 2024 03:11
Export record
Altmetrics
Contributors
Author:
P O Ali
Author:
A J Simpson
Author:
R Allen
Author:
A P Waters
Author:
C J Humphries
Author:
D A Johnston
Author:
D Rollinson
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics