Monoclonal antibodies from Epstein-Barr virus-transformed lymphocytes of common marmosets (Callithrix jacchus) immune to malaria
Monoclonal antibodies from Epstein-Barr virus-transformed lymphocytes of common marmosets (Callithrix jacchus) immune to malaria
The B lymphocytes of the common marmoset Callithrix jacchus can be immortalized by infection with Epstein-Barr virus (EBV) in vitro (Desgranges et al., 1976). C. jacchus is susceptible to infection with the blood stages of several species of malaria parasite including the line designated MVF1 (Mitchell et al., 1988) from which it recovers and shows immunity to reinfection. By exploiting these two phenomena, EBV-transformed, marmoset lymphoblastoid cell lines secreting antibodies to malaria parasite antigens have been generated and cloned. We believe this to be the first time that monoclonal antibodies (MAbs) have been raised from common marmosets. Since numerous and diverse human pathogens can infect this small primate in the laboratory, these methods may prove generally applicable for the generation of MAbs whose specificities derive from immune responses to infection.
Animals, Antibodies, Monoclonal/biosynthesis, Antibodies, Protozoan/biosynthesis, B-Lymphocytes/immunology, Callitrichinae/immunology, Cell Transformation, Viral, Clone Cells, Herpesvirus 4, Human, Malaria/immunology
187-95
Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Knight, A M
60877b54-a1cd-4bc5-8bdc-7b2ecc41d88c
Naylor, B A
bb2b9152-518a-42e0-af74-063aa8d99397
Wedderburn, N
478d50f1-a7bb-479d-9b19-b8030531f77f
Mitchell, G H
f45cae8f-b55d-407c-adc7-34dc7beb36f5
9 March 1990
Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Knight, A M
60877b54-a1cd-4bc5-8bdc-7b2ecc41d88c
Naylor, B A
bb2b9152-518a-42e0-af74-063aa8d99397
Wedderburn, N
478d50f1-a7bb-479d-9b19-b8030531f77f
Mitchell, G H
f45cae8f-b55d-407c-adc7-34dc7beb36f5
Johnston, D A, Knight, A M, Naylor, B A, Wedderburn, N and Mitchell, G H
(1990)
Monoclonal antibodies from Epstein-Barr virus-transformed lymphocytes of common marmosets (Callithrix jacchus) immune to malaria.
Journal of Immunological Methods, 127 (2), .
(doi:10.1016/0022-1759(90)90068-7).
Abstract
The B lymphocytes of the common marmoset Callithrix jacchus can be immortalized by infection with Epstein-Barr virus (EBV) in vitro (Desgranges et al., 1976). C. jacchus is susceptible to infection with the blood stages of several species of malaria parasite including the line designated MVF1 (Mitchell et al., 1988) from which it recovers and shows immunity to reinfection. By exploiting these two phenomena, EBV-transformed, marmoset lymphoblastoid cell lines secreting antibodies to malaria parasite antigens have been generated and cloned. We believe this to be the first time that monoclonal antibodies (MAbs) have been raised from common marmosets. Since numerous and diverse human pathogens can infect this small primate in the laboratory, these methods may prove generally applicable for the generation of MAbs whose specificities derive from immune responses to infection.
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Published date: 9 March 1990
Keywords:
Animals, Antibodies, Monoclonal/biosynthesis, Antibodies, Protozoan/biosynthesis, B-Lymphocytes/immunology, Callitrichinae/immunology, Cell Transformation, Viral, Clone Cells, Herpesvirus 4, Human, Malaria/immunology
Identifiers
Local EPrints ID: 468606
URI: http://eprints.soton.ac.uk/id/eprint/468606
ISSN: 0022-1759
PURE UUID: 371cd29d-53c6-404f-910c-659879bde51d
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Date deposited: 18 Aug 2022 16:42
Last modified: 17 Mar 2024 03:11
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Author:
D A Johnston
Author:
A M Knight
Author:
B A Naylor
Author:
N Wedderburn
Author:
G H Mitchell
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