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Tocotrienols ameliorate neurodegeneration and motor deficits in the 6-OHDA induced rat model of Parkinsonism: Behavioural and Immunohistochemistry Analysis

Tocotrienols ameliorate neurodegeneration and motor deficits in the 6-OHDA induced rat model of Parkinsonism: Behavioural and Immunohistochemistry Analysis
Tocotrienols ameliorate neurodegeneration and motor deficits in the 6-OHDA induced rat model of Parkinsonism: Behavioural and Immunohistochemistry Analysis
Parkinson's disease (PD) is a debilitating neurodegenerative disease, which progresses over time, causing pathological depigmentation of the substantia nigra (SN) in the midbrain due to loss of dopaminergic neurons. Emerging studies revealed the promising effects of some nutrient compounds in reducing the risk of PD. One such nutrient compound that possess neuroprotective effects and prevents neurodegeneration is tocotrienol (T3), a vitamin E family member. In the present study, a single dose intracisternal injection of 250 µg 6-hydroxydopamine (6-OHDA) was used to induce parkinsonism in male Sprague Dawley (SD) rats. Forty-eight hours post injection, the SD rats were orally supplemented with alpha (α)- and gamma (γ)-T3 for 28 days. The neuroprotective effects of α- and γ-T3 were evaluated using behavioural studies and immunohistochemistry (IHC). The findings from this study revealed that supplementation of α- and γ-T3 was able to ameliorate the motor deficits induced by 6-OHDA and improve the neuronal functions by reducing inflammation, reversing the neuronal degradation, and preventing further reduction of dopaminergic neurons in the SN and striatum (STR) fibre density.
2072-6643
Kumari, Mangala
b1c1e6ab-59b0-41dd-8990-1e43508f7cc8
Ramdas, Premdass
4a86e6c9-a322-4749-8724-0e1aacbab46d
Radhakrishnan, Ammu Kutty
d12cd909-ae1c-4024-832e-4199b23b8790
Kutty, Methil Kannan
b84280bd-048e-4fdf-b734-7f652fe25bdc
Haleagrahara, Nagaraja
f366d0d4-71d1-493a-aa52-84884d8101ad
Kumari, Mangala
b1c1e6ab-59b0-41dd-8990-1e43508f7cc8
Ramdas, Premdass
4a86e6c9-a322-4749-8724-0e1aacbab46d
Radhakrishnan, Ammu Kutty
d12cd909-ae1c-4024-832e-4199b23b8790
Kutty, Methil Kannan
b84280bd-048e-4fdf-b734-7f652fe25bdc
Haleagrahara, Nagaraja
f366d0d4-71d1-493a-aa52-84884d8101ad

Kumari, Mangala, Ramdas, Premdass, Radhakrishnan, Ammu Kutty, Kutty, Methil Kannan and Haleagrahara, Nagaraja (2021) Tocotrienols ameliorate neurodegeneration and motor deficits in the 6-OHDA induced rat model of Parkinsonism: Behavioural and Immunohistochemistry Analysis. Nutrients, 13 (5). (doi:10.3390/nu13051583).

Record type: Article

Abstract

Parkinson's disease (PD) is a debilitating neurodegenerative disease, which progresses over time, causing pathological depigmentation of the substantia nigra (SN) in the midbrain due to loss of dopaminergic neurons. Emerging studies revealed the promising effects of some nutrient compounds in reducing the risk of PD. One such nutrient compound that possess neuroprotective effects and prevents neurodegeneration is tocotrienol (T3), a vitamin E family member. In the present study, a single dose intracisternal injection of 250 µg 6-hydroxydopamine (6-OHDA) was used to induce parkinsonism in male Sprague Dawley (SD) rats. Forty-eight hours post injection, the SD rats were orally supplemented with alpha (α)- and gamma (γ)-T3 for 28 days. The neuroprotective effects of α- and γ-T3 were evaluated using behavioural studies and immunohistochemistry (IHC). The findings from this study revealed that supplementation of α- and γ-T3 was able to ameliorate the motor deficits induced by 6-OHDA and improve the neuronal functions by reducing inflammation, reversing the neuronal degradation, and preventing further reduction of dopaminergic neurons in the SN and striatum (STR) fibre density.

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nutrients-13-01583 (1) - Version of Record
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Accepted/In Press date: 7 April 2021
Published date: 10 May 2021

Identifiers

Local EPrints ID: 468677
URI: http://eprints.soton.ac.uk/id/eprint/468677
ISSN: 2072-6643
PURE UUID: 84e9b277-6bfb-4895-84ab-20e6c46cea3f

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Date deposited: 22 Aug 2022 16:44
Last modified: 16 Mar 2024 21:21

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Contributors

Author: Mangala Kumari
Author: Premdass Ramdas
Author: Ammu Kutty Radhakrishnan
Author: Methil Kannan Kutty
Author: Nagaraja Haleagrahara

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