Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling
Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling
BACKGROUND: Parasitic helminths of the genus Schistosoma mate, achieve sexual maturity and produce eggs in the bloodstream of their definitive hosts, and the most important pathological consequences of the infection are associated with this process. We have used cDNA microarray technology to initiate genome-wide gene-expression studies of sex and sexual development in mature Schistosoma mansoni parasites.
RESULTS: An S. mansoni-specific cDNA microarray was fabricated using 576 expressed sequence tags selected from three cDNA libraries and originating from two different parasite developmental stages. Five independent cDNA microarray hybridizations were analyzed using stringent filtering criteria and careful quality control, leading to the identification of 12 new female-associated and 4 new male-associated gene transcripts in the mature adult schistosome. Statistical analysis of variation demonstrated high levels of agreement within a cDNA microarray (correlation coefficient 0.91; median coefficient of variation 11.1%) and between cDNA microarrays (correlation coefficient 0.90; median coefficient of variation 14.4%). RT-PCR analysis confirmed the cDNA microarray results, thereby supporting the reliability of the system.
CONCLUSIONS: Our study expands the list of S. mansoni gender-associated gene transcripts from all previous studies by a factor of two. Among the new associations identified, a tyrosinase ortholog was preferentially expressed in the adult female, and a dynein light-chain ortholog was highly induced in the adult male. cDNA microarrays offer the potential for exponential leaps in the understanding of parasite biology and this study shows how molecules involved in sexual biology can be rapidly identified.
Animals, DNA, Complementary/genetics, DNA, Helminth/genetics, Female, Gene Expression Profiling/methods, Gene Expression Regulation/physiology, Genes, Helminth/genetics, Male, Oligonucleotide Array Sequence Analysis/methods, Polymerase Chain Reaction/methods, Reverse Transcriptase Polymerase Chain Reaction, Schistosoma mansoni/genetics, Sex Characteristics, Transcription, Genetic/physiology
Hoffmann, Karl F.
78436a7e-cf69-4cf5-9ff2-238cb826d502
Johnston, David A.
b41163c9-b9d2-425c-af99-2a357204014e
Dunne, David W
8ca7deaa-0efc-4db1-accc-9c28a305e69f
25 July 2002
Hoffmann, Karl F.
78436a7e-cf69-4cf5-9ff2-238cb826d502
Johnston, David A.
b41163c9-b9d2-425c-af99-2a357204014e
Dunne, David W
8ca7deaa-0efc-4db1-accc-9c28a305e69f
Hoffmann, Karl F., Johnston, David A. and Dunne, David W
(2002)
Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling.
Genome Biology, 3 (8), [Research0041.1].
(doi:10.1186/gb-2002-3-8-research0041).
Abstract
BACKGROUND: Parasitic helminths of the genus Schistosoma mate, achieve sexual maturity and produce eggs in the bloodstream of their definitive hosts, and the most important pathological consequences of the infection are associated with this process. We have used cDNA microarray technology to initiate genome-wide gene-expression studies of sex and sexual development in mature Schistosoma mansoni parasites.
RESULTS: An S. mansoni-specific cDNA microarray was fabricated using 576 expressed sequence tags selected from three cDNA libraries and originating from two different parasite developmental stages. Five independent cDNA microarray hybridizations were analyzed using stringent filtering criteria and careful quality control, leading to the identification of 12 new female-associated and 4 new male-associated gene transcripts in the mature adult schistosome. Statistical analysis of variation demonstrated high levels of agreement within a cDNA microarray (correlation coefficient 0.91; median coefficient of variation 11.1%) and between cDNA microarrays (correlation coefficient 0.90; median coefficient of variation 14.4%). RT-PCR analysis confirmed the cDNA microarray results, thereby supporting the reliability of the system.
CONCLUSIONS: Our study expands the list of S. mansoni gender-associated gene transcripts from all previous studies by a factor of two. Among the new associations identified, a tyrosinase ortholog was preferentially expressed in the adult female, and a dynein light-chain ortholog was highly induced in the adult male. cDNA microarrays offer the potential for exponential leaps in the understanding of parasite biology and this study shows how molecules involved in sexual biology can be rapidly identified.
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More information
Published date: 25 July 2002
Keywords:
Animals, DNA, Complementary/genetics, DNA, Helminth/genetics, Female, Gene Expression Profiling/methods, Gene Expression Regulation/physiology, Genes, Helminth/genetics, Male, Oligonucleotide Array Sequence Analysis/methods, Polymerase Chain Reaction/methods, Reverse Transcriptase Polymerase Chain Reaction, Schistosoma mansoni/genetics, Sex Characteristics, Transcription, Genetic/physiology
Identifiers
Local EPrints ID: 468927
URI: http://eprints.soton.ac.uk/id/eprint/468927
ISSN: 1465-6906
PURE UUID: d1b19ac6-c66a-414c-86c1-a677f6341845
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Date deposited: 01 Sep 2022 16:49
Last modified: 17 Mar 2024 03:11
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Contributors
Author:
Karl F. Hoffmann
Author:
David A. Johnston
Author:
David W Dunne
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