The University of Southampton
University of Southampton Institutional Repository

Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni

Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni
Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni
The genome of Schistosoma mansoni contains a proviral form of a retrovirus-like long terminal repeat (LTR) retrotransposon, designated BOUDICCA: Sequence and structural characterization of the new mobile genetic element, which was found in bacterial artificial chromosomes prepared from S. mansoni genomic DNA, revealed the presence of three putative open reading frames (ORFs) bounded by direct LTRs of 328 bp in length. ORF1 encoded a retrovirus-like major homology region and a Cys/His box motif, also present in Gag polyproteins of related retrotransposons and retroviruses. ORF2 encoded enzymatic domains and motifs characteristic of a retrovirus-like polyprotein, including aspartic protease, reverse transcriptase, RNase H, and integrase, in that order, a domain order similar to that of the gypsy/Ty3 retrotransposons. An additional ORF at the 3' end of the retrotransposon may encode an envelope protein. Phylogenetic comparison based on the reverse transcriptase domain of ORF2 confirmed that Boudicca was a gypsy-like retrotransposon and showed that it was most closely related to CsRn1 from the Oriental liver fluke Clonorchis sinensis and to kabuki from Bombyx mori. Bioinformatics approaches together with Southern hybridization analysis of genomic DNA of S. mansoni and the screening of a bacterial artificial chromosome library representing approximately 8-fold coverage of the S. mansoni genome revealed that numerous copies of Boudicca were interspersed throughout the schistosome genome. By reverse transcription-PCR, mRNA transcripts were detected in the sporocyst, cercaria, and adult developmental stages of S. mansoni, indicating that Boudicca is actively transcribed in this trematode.
Amino Acid Sequence, Animals, Base Sequence, Chromosomes, Artificial, Bacterial, Computational Biology, DNA, Helminth/analysis, Gene Expression Regulation, Developmental, Genome, Genomic Library, Molecular Sequence Data, Phylogeny, Retroelements/genetics, Schistosoma mansoni/genetics, Sequence Analysis, DNA, Transcription, Genetic
0022-538X
6153-66
Copeland, Claudia S
dba2d6f4-29d4-42c1-9271-a1630935a58a
Brindley, Paul J.
6d03751b-772b-47df-b6c1-4cf075fe5eb5
Heyers, Oliver
f154f75a-9072-4ea7-aa63-18b958509114
Michael, Scott F
bc41bcf5-ff54-4860-8125-e3750f222d1a
Johnston, David A
b41163c9-b9d2-425c-af99-2a357204014e
Williams, David L
9c7e4550-faa3-4664-b9cb-22fc69770bd7
Ivens, Alasdair C
f6b6c828-bd07-412f-9c07-f076a2f6ebd1
Kalinna, Bernd H
2fc72349-1967-4626-87f0-41cd79647d23
Copeland, Claudia S
dba2d6f4-29d4-42c1-9271-a1630935a58a
Brindley, Paul J.
6d03751b-772b-47df-b6c1-4cf075fe5eb5
Heyers, Oliver
f154f75a-9072-4ea7-aa63-18b958509114
Michael, Scott F
bc41bcf5-ff54-4860-8125-e3750f222d1a
Johnston, David A
b41163c9-b9d2-425c-af99-2a357204014e
Williams, David L
9c7e4550-faa3-4664-b9cb-22fc69770bd7
Ivens, Alasdair C
f6b6c828-bd07-412f-9c07-f076a2f6ebd1
Kalinna, Bernd H
2fc72349-1967-4626-87f0-41cd79647d23

Copeland, Claudia S, Brindley, Paul J., Heyers, Oliver, Michael, Scott F, Johnston, David A, Williams, David L, Ivens, Alasdair C and Kalinna, Bernd H (2003) Boudicca, a retrovirus-like long terminal repeat retrotransposon from the genome of the human blood fluke Schistosoma mansoni. Journal of Virology, 77 (11), 6153-66. (doi:10.1128/jvi.77.11.6153-6166.2003).

Record type: Article

Abstract

The genome of Schistosoma mansoni contains a proviral form of a retrovirus-like long terminal repeat (LTR) retrotransposon, designated BOUDICCA: Sequence and structural characterization of the new mobile genetic element, which was found in bacterial artificial chromosomes prepared from S. mansoni genomic DNA, revealed the presence of three putative open reading frames (ORFs) bounded by direct LTRs of 328 bp in length. ORF1 encoded a retrovirus-like major homology region and a Cys/His box motif, also present in Gag polyproteins of related retrotransposons and retroviruses. ORF2 encoded enzymatic domains and motifs characteristic of a retrovirus-like polyprotein, including aspartic protease, reverse transcriptase, RNase H, and integrase, in that order, a domain order similar to that of the gypsy/Ty3 retrotransposons. An additional ORF at the 3' end of the retrotransposon may encode an envelope protein. Phylogenetic comparison based on the reverse transcriptase domain of ORF2 confirmed that Boudicca was a gypsy-like retrotransposon and showed that it was most closely related to CsRn1 from the Oriental liver fluke Clonorchis sinensis and to kabuki from Bombyx mori. Bioinformatics approaches together with Southern hybridization analysis of genomic DNA of S. mansoni and the screening of a bacterial artificial chromosome library representing approximately 8-fold coverage of the S. mansoni genome revealed that numerous copies of Boudicca were interspersed throughout the schistosome genome. By reverse transcription-PCR, mRNA transcripts were detected in the sporocyst, cercaria, and adult developmental stages of S. mansoni, indicating that Boudicca is actively transcribed in this trematode.

This record has no associated files available for download.

More information

Published date: 1 June 2003
Keywords: Amino Acid Sequence, Animals, Base Sequence, Chromosomes, Artificial, Bacterial, Computational Biology, DNA, Helminth/analysis, Gene Expression Regulation, Developmental, Genome, Genomic Library, Molecular Sequence Data, Phylogeny, Retroelements/genetics, Schistosoma mansoni/genetics, Sequence Analysis, DNA, Transcription, Genetic

Identifiers

Local EPrints ID: 468940
URI: http://eprints.soton.ac.uk/id/eprint/468940
ISSN: 0022-538X
PURE UUID: 705f8f6a-af3c-4dab-8d5e-2ccf01cf0e6a
ORCID for David A Johnston: ORCID iD orcid.org/0000-0001-6703-6014

Catalogue record

Date deposited: 01 Sep 2022 17:01
Last modified: 02 Sep 2022 01:41

Export record

Altmetrics

Contributors

Author: Claudia S Copeland
Author: Paul J. Brindley
Author: Oliver Heyers
Author: Scott F Michael
Author: David A Johnston ORCID iD
Author: David L Williams
Author: Alasdair C Ivens
Author: Bernd H Kalinna

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×