Restriction enzyme mapping of ribosomal DNA of Schistosoma spindale and S. leiperi (Digenea) and its application to interspecific differentiation
Restriction enzyme mapping of ribosomal DNA of Schistosoma spindale and S. leiperi (Digenea) and its application to interspecific differentiation
Restriction maps were determined for the ribosomal RNA gene complex of Schistosoma spindale and S. leiperi. The restriction map of the S. spindale rRNA gene complex was found to differ from those of other schistosomes previously described by the presence of an additional EcoRI site in the non transcribed spacer region. In common with S. mattheei and S. margrebowiei, both S. leiperi and S. spindale appear to have insertions in the transcribed spacer region, relative to S. mansoni. EcoRI digests of genomic DNA, probed with pSM 889, enabled differentiation of S. spindale and S. leiperi from four other species of schistosome.
13-17
Kaukas, A
d00995f5-f483-4010-b38d-e9cbfe417487
Johnston, DA
b41163c9-b9d2-425c-af99-2a357204014e
Kane, RA
8abb698c-91f2-4eaa-9db5-27a0ea0d6525
Rollinson, D
92dc2a29-b518-4968-9e30-261fa808a342
1 January 1994
Kaukas, A
d00995f5-f483-4010-b38d-e9cbfe417487
Johnston, DA
b41163c9-b9d2-425c-af99-2a357204014e
Kane, RA
8abb698c-91f2-4eaa-9db5-27a0ea0d6525
Rollinson, D
92dc2a29-b518-4968-9e30-261fa808a342
Kaukas, A, Johnston, DA, Kane, RA and Rollinson, D
(1994)
Restriction enzyme mapping of ribosomal DNA of Schistosoma spindale and S. leiperi (Digenea) and its application to interspecific differentiation.
Systematic Parasitology, 27 (1), .
(doi:10.1007/BF02185664).
Abstract
Restriction maps were determined for the ribosomal RNA gene complex of Schistosoma spindale and S. leiperi. The restriction map of the S. spindale rRNA gene complex was found to differ from those of other schistosomes previously described by the presence of an additional EcoRI site in the non transcribed spacer region. In common with S. mattheei and S. margrebowiei, both S. leiperi and S. spindale appear to have insertions in the transcribed spacer region, relative to S. mansoni. EcoRI digests of genomic DNA, probed with pSM 889, enabled differentiation of S. spindale and S. leiperi from four other species of schistosome.
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Published date: 1 January 1994
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Local EPrints ID: 468946
URI: http://eprints.soton.ac.uk/id/eprint/468946
ISSN: 0165-5752
PURE UUID: cbe121cb-2344-4d34-a407-738790e53fe8
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Date deposited: 01 Sep 2022 17:05
Last modified: 17 Mar 2024 03:11
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Author:
A Kaukas
Author:
DA Johnston
Author:
RA Kane
Author:
D Rollinson
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