Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy
Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy
Bacterial mechanical properties (cell wall stiffness and turgor) are important factors for bacterial survival in harsh environments. For an individual bacterial cell, it is challenging to determine the cell wall stiffness and turgor simultaneously. In this study, we adopted a combined finite element modelling and mathematical modelling approach to simultaneously determine bacterial cell wall stiffness and turgor of an individual bacterial cell based on atomic force microscopy (AFM) nanoindentation. The mechanical properties and turgor of Staphylococcus epidermidis, determined by our method are consistent with other independent studies. For a given aqueous environment, bacterial cell wall stiffness increased linearly with an increase in turgor. Higher osmolarity leads to a decrease in both cell wall stiffness and turgor. We also demonstrated that the change of turgor is associated with a change in viscosity of the bacterial cell
12060-12068
Han, Rui
57af6baf-6a3b-40f7-ae28-db89cd3575f8
Vollmer, Waldemar
9e7142d0-635f-43da-a483-de7fb7e9669a
Perry, John
1ab15608-bec6-4a0a-86ad-a9beb1f59e14
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Chen, Jinju
105a3320-0a27-49fc-83c5-9c07e89b506c
25 August 2022
Han, Rui
57af6baf-6a3b-40f7-ae28-db89cd3575f8
Vollmer, Waldemar
9e7142d0-635f-43da-a483-de7fb7e9669a
Perry, John
1ab15608-bec6-4a0a-86ad-a9beb1f59e14
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Chen, Jinju
105a3320-0a27-49fc-83c5-9c07e89b506c
Han, Rui, Vollmer, Waldemar, Perry, John, Stoodley, Paul and Chen, Jinju
(2022)
Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy.
Journal of Materials Research, 14 (33), .
(doi:10.1039/D2NR02577A).
Abstract
Bacterial mechanical properties (cell wall stiffness and turgor) are important factors for bacterial survival in harsh environments. For an individual bacterial cell, it is challenging to determine the cell wall stiffness and turgor simultaneously. In this study, we adopted a combined finite element modelling and mathematical modelling approach to simultaneously determine bacterial cell wall stiffness and turgor of an individual bacterial cell based on atomic force microscopy (AFM) nanoindentation. The mechanical properties and turgor of Staphylococcus epidermidis, determined by our method are consistent with other independent studies. For a given aqueous environment, bacterial cell wall stiffness increased linearly with an increase in turgor. Higher osmolarity leads to a decrease in both cell wall stiffness and turgor. We also demonstrated that the change of turgor is associated with a change in viscosity of the bacterial cell
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Accepted/In Press date: 6 August 2022
Published date: 25 August 2022
Additional Information:
Funding Information:
J. Chen acknowledges funding from the Engineering and Physical Sciences Research Council (EP/R025606/1; EP/V049615/1). W. Vollmer was supported by the UKRI Strategic Priorities Fund ( https://www.ukri.org ) EP/T002778/1. R. Han acknowledges the PhD scholarship from Chinese Scholarship Council and Newcastle University. Prof. NS Jakubovic is acknowledged for providing the bacteria used for this study. We thank Ross Laws, Tracey Davey and Yufeng Zhu for their support on SEM imaging. We also acknowledge Prof. Glen McHale and Mr Jack Dawson for careful proofreading.
Publisher Copyright:
© 2022 The Royal Society of Chemistry.
Identifiers
Local EPrints ID: 468993
URI: http://eprints.soton.ac.uk/id/eprint/468993
ISSN: 0884-2914
PURE UUID: 024a41b5-04ae-4d48-954b-6c60acc7a00f
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Date deposited: 02 Sep 2022 19:13
Last modified: 17 Mar 2024 03:18
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Contributors
Author:
Rui Han
Author:
Waldemar Vollmer
Author:
John Perry
Author:
Jinju Chen
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