Improved method of total histone isolation from Arabidopsis thaliana
Improved method of total histone isolation from Arabidopsis thaliana
Standard methods of isolation of chromatin histones from Arabdopsis thaliana yield strongly degraded proteins when applied to plants grown from seeds in axenic liquid media. For isolation of undegraded histones from Arabidopsis grown in liquid media we used extraction with guanidine hydrochloride followed by selective binding of histones on BioRex 70 resin in the batch system. The quality of obtained proteins is confirmed by SDS polyacrylamide gel electrophoresis.
quanidine hydrochloride, SDS PAGE
299-302
Przewloka, M. R.
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Jerzmanowski, A.
fd267f9e-942e-4f03-a56a-db15ad119bd6
1997
Przewloka, M. R.
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Jerzmanowski, A.
fd267f9e-942e-4f03-a56a-db15ad119bd6
Przewloka, M. R. and Jerzmanowski, A.
(1997)
Improved method of total histone isolation from Arabidopsis thaliana.
Biologia Plantarum, 39 (2), .
(doi:10.1023/A:1000688117822).
Abstract
Standard methods of isolation of chromatin histones from Arabdopsis thaliana yield strongly degraded proteins when applied to plants grown from seeds in axenic liquid media. For isolation of undegraded histones from Arabidopsis grown in liquid media we used extraction with guanidine hydrochloride followed by selective binding of histones on BioRex 70 resin in the batch system. The quality of obtained proteins is confirmed by SDS polyacrylamide gel electrophoresis.
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Published date: 1997
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Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
Keywords:
quanidine hydrochloride, SDS PAGE
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Local EPrints ID: 469038
URI: http://eprints.soton.ac.uk/id/eprint/469038
ISSN: 0006-3134
PURE UUID: 061d27e8-2ab4-49f0-be18-3f8223b19b81
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Date deposited: 05 Sep 2022 16:58
Last modified: 17 Mar 2024 03:41
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Author:
A. Jerzmanowski
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