Small subunit (18S) ribosomal RNA gene divergence in the genus Schistosoma
Small subunit (18S) ribosomal RNA gene divergence in the genus Schistosoma
An entire 18S rRNA gene sequence from Schistosoma spindale (1990 bases) and partial 18S rRNA gene sequences from S. haematobium (1950 bases) and S. japonicum (1648 bases) have been determined. Together with the previously published sequence of the S. mansoni 18S rRNA gene, these data encompass the 4 recognized Schistosoma species groups. Although Schistosoma 18S rRNA genes are highly conserved, the sequences permit a preliminary molecular phylogeny to be established for the genus. This identifies S. haematobium and S. spindale as sister taxa in a clade with S. mansoni. S. japonicum does not appear to be closely related to this clade. Much of the observed variation occurs within a 'hypervariable' stretch of the gene corresponding to part of the V4 region of 18S rRNA. Despite this variation, the 3 new sequences fit models of 18S rRNA secondary structure predicted from the S. mansoni sequence.
Animals, Base Sequence, Cloning, Molecular, DNA Primers/chemistry, DNA, Ribosomal/chemistry, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, RNA, Ribosomal, 18S/chemistry, Restriction Mapping, Schistosoma/classification, Schistosoma haematobium/classification, Schistosoma japonicum/classification
147-156
Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Kane, R A
28611046-0fdd-4bee-a16c-6e46d5da776e
Rollinson, D
917d0e09-176f-4058-89ff-ec2ad3a496ad
August 1993
Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Kane, R A
28611046-0fdd-4bee-a16c-6e46d5da776e
Rollinson, D
917d0e09-176f-4058-89ff-ec2ad3a496ad
Johnston, D A, Kane, R A and Rollinson, D
(1993)
Small subunit (18S) ribosomal RNA gene divergence in the genus Schistosoma.
Parasitology, 107 ( Pt 2), .
(doi:10.1017/s0031182000067251).
Abstract
An entire 18S rRNA gene sequence from Schistosoma spindale (1990 bases) and partial 18S rRNA gene sequences from S. haematobium (1950 bases) and S. japonicum (1648 bases) have been determined. Together with the previously published sequence of the S. mansoni 18S rRNA gene, these data encompass the 4 recognized Schistosoma species groups. Although Schistosoma 18S rRNA genes are highly conserved, the sequences permit a preliminary molecular phylogeny to be established for the genus. This identifies S. haematobium and S. spindale as sister taxa in a clade with S. mansoni. S. japonicum does not appear to be closely related to this clade. Much of the observed variation occurs within a 'hypervariable' stretch of the gene corresponding to part of the V4 region of 18S rRNA. Despite this variation, the 3 new sequences fit models of 18S rRNA secondary structure predicted from the S. mansoni sequence.
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Published date: August 1993
Keywords:
Animals, Base Sequence, Cloning, Molecular, DNA Primers/chemistry, DNA, Ribosomal/chemistry, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, RNA, Ribosomal, 18S/chemistry, Restriction Mapping, Schistosoma/classification, Schistosoma haematobium/classification, Schistosoma japonicum/classification
Identifiers
Local EPrints ID: 469212
URI: http://eprints.soton.ac.uk/id/eprint/469212
ISSN: 0031-1820
PURE UUID: 57d29c6f-14de-4553-8d6e-fad37b585b87
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Date deposited: 09 Sep 2022 16:37
Last modified: 17 Mar 2024 03:11
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Author:
D A Johnston
Author:
R A Kane
Author:
D Rollinson
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