High thermostability improves neutralizing antibody responses induced by native-like HIV-1 envelope trimers

e-pub ahead of print date: 28 February 2022

Published date: 28 February 2022

Additional Information: Funding Information: The authors thank Marit van Gils for donating the 10A monoclonal antibody; and Michel Nussenzweig, Hermann Katinger, Mark Connors, James Robinson, Dennis Burton, John Mascola, Peter Kwong, and William Olson for donating antibodies and reagents directly or through the NIH AIDS Research and Reference Reagent Program. We thank Dietmar Katinger and Ehsan Suleiman for providing the squalene emulsion adjuvant. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 681137 (to R.W.S., Q.S., and M.C.). This work was also supported by the U.S. National Institutes of Health Grant P01 AI110657 (to A.B.W. and R.W.S.) and NIAID Contract #HHSN27201100016C (to D.C.M.); by the International AIDS Vaccine Initiative (IAVI); by the Bill and Melinda Gates Foundation through the Collaboration for AIDS Vaccine Discovery (CAVD), grants OPP1111923 and OPP1132237 (to R.W.S.) and OPP1115782 (A.B.W.); by the Aids Fonds Netherlands, Grant #2016019 (to R.W.S.); and by the Fondation Dormeur, Vaduz (to R.W.S.). R.W.S. is a recipient of a Vici grant from the Netherlands Organization for Scientific Research (NWO). We thank EMBO for the Short-Term Fellowship (STS-7814) awarded to S.K. The electron microscopy data were collected at Electron Microscopy Facility of The Scripps Research Institute. Funding Information: The authors thank Marit van Gils for donating the 10A monoclonal antibody; and Michel Nussenzweig, Hermann Katinger, Mark Connors, James Robinson, Dennis Burton, John Mascola, Peter Kwong, and William Olson for donating antibodies and reagents directly or through the NIH AIDS Research and Reference Reagent Program. We thank Dietmar Katinger and Ehsan Suleiman for providing the squalene emulsion adjuvant. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 681137 (to R.W.S., Q.S., and M.C.). This work was also supported by the U.S. National Institutes of Health Grant P01 AI110657 (to A.B.W. and R.W.S.) and NIAID Contract #HHSN27201100016C (to D.C.M.); by the International AIDS Vaccine Initiative (IAVI); by the Bill and Melinda Gates Foundation through the Collaboration for AIDS Vaccine Discovery (CAVD), grants OPP1111923 and OPP1132237 (to R.W.S.) and OPP1115782 (A.B.W.); by the Aids Fonds Netherlands, Grant #2016019 (to R.W.S.); and by the Fondation Dormeur, Vaduz (to R.W.S.). R.W.S. is a recipient of a Vici grant from the Netherlands Organization for Scientific Research (NWO). We thank EMBO for the Short-Term Fellowship (STS-7814) awarded to S.K. The electron microscopy data were collected at Electron Microscopy Facility of The Scripps Research Institute. Publisher Copyright: © 2022, The Author(s).

Local EPrints ID: 469241

URI: http://eprints.soton.ac.uk/id/eprint/469241

DOI: doi:10.1038/s41541-022-00446-4

ISSN: 2059-0105

PURE UUID: 56d2a65c-5fd7-4e81-bcb3-87946366c5df

ORCID for Max Crispin: orcid.org/0000-0002-1072-2694

Date deposited: 09 Sep 2022 16:48

Last modified: 17 Mar 2024 03:47