Resin bead micro-UV - visible absorption spectroscopy
Resin bead micro-UV - visible absorption spectroscopy
The construction and design of a microscope coupled with a miniature UV-vis spectrometer is described. This was applied to the study of dyes linked to solid supports and displayed good correlation in spectral shape and λmax values when compared to the dyes in solution, as well as showing a linear relationship between dye loading and UV-vis absorbance. The spectral profiles of these dyes at various pH's were measured and used to determine the pK(a) of the dyes on the beads, which were compared with the pK(a) values of the dyes in solution, thus enabling the dye-loaded beads to act as pH sensors.
2247-2251
Wong, Lu Shin
9b2d2158-75d7-4b67-9194-412345616995
Birembaut, Fabrice
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Brocklesby, William S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
March 2005
Wong, Lu Shin
9b2d2158-75d7-4b67-9194-412345616995
Birembaut, Fabrice
d60a2b6f-001c-4b9d-87b3-bb426085b51d
Brocklesby, William S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
Wong, Lu Shin, Birembaut, Fabrice, Brocklesby, William S., Frey, Jeremy G. and Bradley, Mark
(2005)
Resin bead micro-UV - visible absorption spectroscopy.
Analytical Chemistry, 77 (7), .
(doi:10.1021/ac049319i).
Abstract
The construction and design of a microscope coupled with a miniature UV-vis spectrometer is described. This was applied to the study of dyes linked to solid supports and displayed good correlation in spectral shape and λmax values when compared to the dyes in solution, as well as showing a linear relationship between dye loading and UV-vis absorbance. The spectral profiles of these dyes at various pH's were measured and used to determine the pK(a) of the dyes on the beads, which were compared with the pK(a) values of the dyes in solution, thus enabling the dye-loaded beads to act as pH sensors.
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Published date: March 2005
Organisations:
Chemistry
Identifiers
Local EPrints ID: 46947
URI: http://eprints.soton.ac.uk/id/eprint/46947
ISSN: 0003-2700
PURE UUID: fda3ad37-1192-48c9-bb5f-b80cb1d861f9
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Date deposited: 25 Jul 2007
Last modified: 16 Mar 2024 02:39
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Author:
Lu Shin Wong
Author:
Fabrice Birembaut
Author:
Mark Bradley
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