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Resin bead micro-UV - visible absorption spectroscopy

Resin bead micro-UV - visible absorption spectroscopy
Resin bead micro-UV - visible absorption spectroscopy
The construction and design of a microscope coupled with a miniature UV-vis spectrometer is described. This was applied to the study of dyes linked to solid supports and displayed good correlation in spectral shape and lambda(max) values when compared to the dyes in solution, as well as showing a linear relationship between dye loading and UV-vis absorbance. The spectral profiles of these dyes at various pH's were measured and used to determine the pK(a) of the dyes on the beads, which were compared with the pK(a) values of the dyes in solution, thus enabling the dye-loaded beads to act as pH sensors.
0003-2700
2247-2251
Wong, Lu Shin
9b2d2158-75d7-4b67-9194-412345616995
Birembaut, Fabrice
d60a2b6f-001c-4b9d-87b3-bb426085b51d
Brocklesby, William S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
Wong, Lu Shin
9b2d2158-75d7-4b67-9194-412345616995
Birembaut, Fabrice
d60a2b6f-001c-4b9d-87b3-bb426085b51d
Brocklesby, William S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900

Wong, Lu Shin, Birembaut, Fabrice, Brocklesby, William S., Frey, Jeremy G. and Bradley, Mark (2005) Resin bead micro-UV - visible absorption spectroscopy. Analytical Chemistry, 77 (7), 2247-2251. (doi:10.1021/ac049319i).

Record type: Article

Abstract

The construction and design of a microscope coupled with a miniature UV-vis spectrometer is described. This was applied to the study of dyes linked to solid supports and displayed good correlation in spectral shape and lambda(max) values when compared to the dyes in solution, as well as showing a linear relationship between dye loading and UV-vis absorbance. The spectral profiles of these dyes at various pH's were measured and used to determine the pK(a) of the dyes on the beads, which were compared with the pK(a) values of the dyes in solution, thus enabling the dye-loaded beads to act as pH sensors.

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More information

Published date: March 2005
Organisations: Chemistry

Identifiers

Local EPrints ID: 46947
URI: https://eprints.soton.ac.uk/id/eprint/46947
ISSN: 0003-2700
PURE UUID: fda3ad37-1192-48c9-bb5f-b80cb1d861f9
ORCID for William S. Brocklesby: ORCID iD orcid.org/0000-0002-2123-6712
ORCID for Jeremy G. Frey: ORCID iD orcid.org/0000-0003-0842-4302

Catalogue record

Date deposited: 25 Jul 2007
Last modified: 18 Jul 2019 01:26

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