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Oxygen microoptodes: a new tool for oxygen measurements in aquatic animal ecology

Oxygen microoptodes: a new tool for oxygen measurements in aquatic animal ecology
Oxygen microoptodes: a new tool for oxygen measurements in aquatic animal ecology
We describe two applications of a recently introduced system for very precise, continuous measurement of water oxygen saturation. Oxygen microoptodes (based on the dynamic fluorescence quenching principle) with a tip diameter of ~50 µm, an eight-channel optode array, an intermittent flow system, and online data registration were used to perform two types of experiments. The metabolic activity of Antarctic invertebrates (sponges and scallops) was estimated in respiration experiments, and, secondly, oxygen saturation inside living sponge tissue was determined in different flow regimes. Even in long-term experiments (several days) no drift was detectable in between calibrations. Data obtained were in excellent correspondence with control measurements performed with a modified Winkler method. Antarctic invertebrates in our study showed low oxygen consumption rates, ranging from 0.03-0.19 cm3 O2 h-1 ind.-1. Oxygen saturation inside living sponge specimens was affected by flow regime and culturing conditions of sponges. Our results suggest that oxygen optodes are a reliable tool for oxygen measurements beyond the methodological limits of traditional methods.
0025-3162
1075-1085
Gatti, S.
5d0d2a0f-83cc-4768-af58-a836751737f0
Brey, T.
721b07df-2ec0-49dd-8780-18bcb7ca28c4
Müller, W.E.G.
a4859875-b6e3-4e31-bb61-dcf5927abe36
Heilmayer, O.
cbad0ced-292f-41ad-a42f-7ff810750457
Holst, G.
555766b0-d016-4517-a836-0b3dc874ade9
Gatti, S.
5d0d2a0f-83cc-4768-af58-a836751737f0
Brey, T.
721b07df-2ec0-49dd-8780-18bcb7ca28c4
Müller, W.E.G.
a4859875-b6e3-4e31-bb61-dcf5927abe36
Heilmayer, O.
cbad0ced-292f-41ad-a42f-7ff810750457
Holst, G.
555766b0-d016-4517-a836-0b3dc874ade9

Gatti, S., Brey, T., Müller, W.E.G., Heilmayer, O. and Holst, G. (2002) Oxygen microoptodes: a new tool for oxygen measurements in aquatic animal ecology. Marine Biology, 140 (6), 1075-1085. (doi:10.1007/s00227-002-0786-9).

Record type: Article

Abstract

We describe two applications of a recently introduced system for very precise, continuous measurement of water oxygen saturation. Oxygen microoptodes (based on the dynamic fluorescence quenching principle) with a tip diameter of ~50 µm, an eight-channel optode array, an intermittent flow system, and online data registration were used to perform two types of experiments. The metabolic activity of Antarctic invertebrates (sponges and scallops) was estimated in respiration experiments, and, secondly, oxygen saturation inside living sponge tissue was determined in different flow regimes. Even in long-term experiments (several days) no drift was detectable in between calibrations. Data obtained were in excellent correspondence with control measurements performed with a modified Winkler method. Antarctic invertebrates in our study showed low oxygen consumption rates, ranging from 0.03-0.19 cm3 O2 h-1 ind.-1. Oxygen saturation inside living sponge specimens was affected by flow regime and culturing conditions of sponges. Our results suggest that oxygen optodes are a reliable tool for oxygen measurements beyond the methodological limits of traditional methods.

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Published date: 2002

Identifiers

Local EPrints ID: 46967
URI: http://eprints.soton.ac.uk/id/eprint/46967
ISSN: 0025-3162
PURE UUID: a49ce8fd-8e51-47a5-9707-0899406f5e64

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Date deposited: 20 Jul 2007
Last modified: 09 Nov 2021 08:58

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Contributors

Author: S. Gatti
Author: T. Brey
Author: W.E.G. Müller
Author: O. Heilmayer
Author: G. Holst

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