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Therapeutic effect of curcumin supplementation in the modulation of NF-kB responsive genes in a collagen-induced arthritis rat model

Therapeutic effect of curcumin supplementation in the modulation of NF-kB responsive genes in a collagen-induced arthritis rat model
Therapeutic effect of curcumin supplementation in the modulation of NF-kB responsive genes in a collagen-induced arthritis rat model
Aims: To assess the therapeutic effect of curcumin supplementation in modulating the expression of NF-κB in the joints of collagen-induced arthritis (CIA) rats.
Place and Duration of Study: Department of Postgraduate Studies and Research, International Medical University, between July 2011 and May 2012.
Methodology: Arthritis was induced in each group of Dark Agouti (DA) rats, by intradermal injection with collagen emulsified in complete Freund’s adjuvant. Treatment groups which were induced with CIA were treated with: 500 mg/kg curcumin; 1000 mg/kg curcumin; 2000 mg/kg curcumin; 25 mg/kg aspirin. Combination treatment groups which were induced with CIA were treated with: 500 mg/kg curcumin and 25 mg/kg aspirin; 1000 mg/kg curcumin and 25 mg/kg aspirin; 2000 mg/kg curcumin and 25 mg/kg aspirin from day 25 to 38. Efficacy was assessed based on ability to reduce paw oedema, histopathological changes, NF-κB expression, serum tumour necrosis factor alpha (TNF-α), interleukin 1-beta (IL-1β) and gluthathione peroxidase (GPx) levels.
Results: Based on histopathological study, immunohistochemical scoring of NF-κB and ELISA analysis of TNF-α, IL-1β and GPx levels, our study found that curcumin given after arthritis in high doses, shows effects of healing and this results were comparable to positive control group, which is the arthritic group treated with 25 mg/kg aspirin. Curcumin given in combination with aspirin, showed better reduction in pathology in arthritic group compared to positive control group, especially with higher doses of curcumin.
Conclusion: Curcumin was effective in reducing inflammatory changes seen in CIA joints which were proved through histopathological, immunohistochemical and biochemical analysis, however only at high doses.
2456-8899
2940-2954
Somasundaram, Shanmugam
cc3b01e6-4a1b-4a6f-bfe9-597930895152
Chakravarthi, Sirkumar
ff58b33c-edbd-41cb-8997-2445821a5c35
Radakrishnan, Ammu
aa14ebf1-1098-48b4-8533-c066f4d5c55f
Ramdas, Premdass
4a86e6c9-a322-4749-8724-0e1aacbab46d
Haleagrahara, Nagaraja
ab8f74a0-4a4b-4c05-a332-4def5a0a337a
Kumari, Mangala
b1c1e6ab-59b0-41dd-8990-1e43508f7cc8
Somasundaram, Shanmugam
cc3b01e6-4a1b-4a6f-bfe9-597930895152
Chakravarthi, Sirkumar
ff58b33c-edbd-41cb-8997-2445821a5c35
Radakrishnan, Ammu
aa14ebf1-1098-48b4-8533-c066f4d5c55f
Ramdas, Premdass
4a86e6c9-a322-4749-8724-0e1aacbab46d
Haleagrahara, Nagaraja
ab8f74a0-4a4b-4c05-a332-4def5a0a337a
Kumari, Mangala
b1c1e6ab-59b0-41dd-8990-1e43508f7cc8

Somasundaram, Shanmugam, Chakravarthi, Sirkumar, Radakrishnan, Ammu, Ramdas, Premdass, Haleagrahara, Nagaraja and Kumari, Mangala (2014) Therapeutic effect of curcumin supplementation in the modulation of NF-kB responsive genes in a collagen-induced arthritis rat model. Journal of Advances in Medicine and Medical Research (JAMMR), 4 (15), 2940-2954. (doi:10.9734/BJMMR/2014/7475).

Record type: Article

Abstract

Aims: To assess the therapeutic effect of curcumin supplementation in modulating the expression of NF-κB in the joints of collagen-induced arthritis (CIA) rats.
Place and Duration of Study: Department of Postgraduate Studies and Research, International Medical University, between July 2011 and May 2012.
Methodology: Arthritis was induced in each group of Dark Agouti (DA) rats, by intradermal injection with collagen emulsified in complete Freund’s adjuvant. Treatment groups which were induced with CIA were treated with: 500 mg/kg curcumin; 1000 mg/kg curcumin; 2000 mg/kg curcumin; 25 mg/kg aspirin. Combination treatment groups which were induced with CIA were treated with: 500 mg/kg curcumin and 25 mg/kg aspirin; 1000 mg/kg curcumin and 25 mg/kg aspirin; 2000 mg/kg curcumin and 25 mg/kg aspirin from day 25 to 38. Efficacy was assessed based on ability to reduce paw oedema, histopathological changes, NF-κB expression, serum tumour necrosis factor alpha (TNF-α), interleukin 1-beta (IL-1β) and gluthathione peroxidase (GPx) levels.
Results: Based on histopathological study, immunohistochemical scoring of NF-κB and ELISA analysis of TNF-α, IL-1β and GPx levels, our study found that curcumin given after arthritis in high doses, shows effects of healing and this results were comparable to positive control group, which is the arthritic group treated with 25 mg/kg aspirin. Curcumin given in combination with aspirin, showed better reduction in pathology in arthritic group compared to positive control group, especially with higher doses of curcumin.
Conclusion: Curcumin was effective in reducing inflammatory changes seen in CIA joints which were proved through histopathological, immunohistochemical and biochemical analysis, however only at high doses.

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More information

Published date: 10 March 2014
Additional Information: Copyright: Somasundaram et al.

Identifiers

Local EPrints ID: 469917
URI: http://eprints.soton.ac.uk/id/eprint/469917
ISSN: 2456-8899
PURE UUID: e6c7fabe-8ad8-46a0-a299-a65ac13270fa

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Date deposited: 28 Sep 2022 17:02
Last modified: 16 Mar 2024 21:22

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Contributors

Author: Shanmugam Somasundaram
Author: Sirkumar Chakravarthi
Author: Ammu Radakrishnan
Author: Premdass Ramdas
Author: Nagaraja Haleagrahara
Author: Mangala Kumari

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