Myo-inositol moderates maternal BMI and glycemia related variations in in-vitro placental C-13-DHA-metabolism, altering their relationships with birthweight
Myo-inositol moderates maternal BMI and glycemia related variations in in-vitro placental C-13-DHA-metabolism, altering their relationships with birthweight
Transplacental docosahexaenoic-acid (DHA) supply for fetal development is regulated by placental DHA-lipid metabolism. Both maternal diabetes and obesity are linked to possible decreased fetal circulating DHA and increased placental DHA-lipids. Since myo-inositol is a promising intervention for gestational diabetes (GDM), we aimed to determine whether myo-inositol could rectify perturbations in placental DHA metabolism associated with maternal increasing glycemia and obesity and examine links with birthweight. Term placental villous explants from 17 women representing a range of BMIs and mid-gestational glycemia, were incubated with 13C-labeled-DHA for 48 h, in 0.3 µmol/L (control) or 60 µmol/L myo-inositol. Individual newly synthesized 13C-DHA-labeled lipid species were quantified by liquid-chromatography-mass-spectrometry. Compared with controls, incubation with myo-inositol decreased most 13C-DHA-lipids in placental explants from women with higher BMI or higher glycemia, but increased 13C-DHA-lipids with normal BMI or lower glycemia. Myo-inositol also increased 13C-DHA-labeled lipids in cases of lower birthweight centile, but induced decreases at higher centiles. Myo-inositol therefore lowered DHA-lipids in placenta with high basal placental DHA-lipid production (higher BMI and glycemia) but increased DHA-lipids where basal processing capacity is low. Myo-inositol thus moderates placental DHA metabolism towards a physiological mean which may in turn moderate birthweight.
Watkins, Oliver C.
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Selvam, Preben
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Pillai, Reshma Appukuttan
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Cracknell-Hazra, Victoria K B
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Yong, Hannah E.J.
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Sharma, Neha
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Bendt, Anne K.
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Godfrey, Keith M.
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Lewis, Rohan M
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Wenk, Markus R.
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Chan, Shiao-Yng
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Watkins, Oliver C.
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Selvam, Preben
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Pillai, Reshma Appukuttan
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Cracknell-Hazra, Victoria K B
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Yong, Hannah E.J.
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Sharma, Neha
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Cazenave-Gassiot, Amaury
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Bendt, Anne K.
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Godfrey, Keith M.
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Lewis, Rohan M
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Wenk, Markus R.
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Chan, Shiao-Yng
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Watkins, Oliver C., Selvam, Preben, Pillai, Reshma Appukuttan, Cracknell-Hazra, Victoria K B, Yong, Hannah E.J., Sharma, Neha, Cazenave-Gassiot, Amaury, Bendt, Anne K., Godfrey, Keith M., Lewis, Rohan M, Wenk, Markus R. and Chan, Shiao-Yng
(2022)
Myo-inositol moderates maternal BMI and glycemia related variations in in-vitro placental C-13-DHA-metabolism, altering their relationships with birthweight.
Scientific Reports, 12 (1), [14895].
(doi:10.1038/s41598-022-18309-2).
Abstract
Transplacental docosahexaenoic-acid (DHA) supply for fetal development is regulated by placental DHA-lipid metabolism. Both maternal diabetes and obesity are linked to possible decreased fetal circulating DHA and increased placental DHA-lipids. Since myo-inositol is a promising intervention for gestational diabetes (GDM), we aimed to determine whether myo-inositol could rectify perturbations in placental DHA metabolism associated with maternal increasing glycemia and obesity and examine links with birthweight. Term placental villous explants from 17 women representing a range of BMIs and mid-gestational glycemia, were incubated with 13C-labeled-DHA for 48 h, in 0.3 µmol/L (control) or 60 µmol/L myo-inositol. Individual newly synthesized 13C-DHA-labeled lipid species were quantified by liquid-chromatography-mass-spectrometry. Compared with controls, incubation with myo-inositol decreased most 13C-DHA-lipids in placental explants from women with higher BMI or higher glycemia, but increased 13C-DHA-lipids with normal BMI or lower glycemia. Myo-inositol also increased 13C-DHA-labeled lipids in cases of lower birthweight centile, but induced decreases at higher centiles. Myo-inositol therefore lowered DHA-lipids in placenta with high basal placental DHA-lipid production (higher BMI and glycemia) but increased DHA-lipids where basal processing capacity is low. Myo-inositol thus moderates placental DHA metabolism towards a physiological mean which may in turn moderate birthweight.
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s41598-022-18309-2
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e-pub ahead of print date: 1 September 2022
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Funding
This research is supported by Clinician Scientist Awards awarded to SYC from the Singapore National Medical Research Council (NMRC/CSA-INV/0010/2016, MOH-CSAINV19nov-0002), by the National University of Singapore, National University Health System Singapore and the Singapore Institute for Clinical Sciences A*STAR. The Singapore Lipidomics Incubator receives funding from the Life Sciences Institute, the National University of Singapore Yong Loo Lin School of Medicine, the National Research Foundation (grant number NRFI2015-05) and A*STAR (IAF-ICP I1901E0040). Funders played no role in study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication.
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Local EPrints ID: 470346
URI: http://eprints.soton.ac.uk/id/eprint/470346
ISSN: 2045-2322
PURE UUID: c484a8de-d225-4537-91fe-797efcd44f31
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Date deposited: 06 Oct 2022 17:03
Last modified: 17 Mar 2024 02:53
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Contributors
Author:
Oliver C. Watkins
Author:
Preben Selvam
Author:
Reshma Appukuttan Pillai
Author:
Victoria K B Cracknell-Hazra
Author:
Hannah E.J. Yong
Author:
Neha Sharma
Author:
Amaury Cazenave-Gassiot
Author:
Anne K. Bendt
Author:
Markus R. Wenk
Author:
Shiao-Yng Chan
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