Studies on locus content mapping
Studies on locus content mapping
Locus content maps are derived from monosomic or disomic chromosomes broken by radiation, shearing, or other clastogen, the fragments being distributed among clones by dilution or incorporation into the cells of another species and scored for segregation of markers. Locus content maps provide evidence about radiosensitivity of chromosome regions, support for order, and approximate location. Omission of the most aberrant and least informative clones increases efficiency of localization. Correct analysis must allow for preferential retention of certain sequences, monosomy or polysomy of donor chromosomes, and error filtration. Combination of these refinements extracts substantially more information from fewer clones. Because of unmodeled peculiarities in the data, the best analysis does not recover the physical map but roughly localizes markers that may be monomorphic and therefore unsuitable for linkage mapping. As with linkage for polymorphic loci, distance in the composite map should be confirmed by physical methods
11814-11818
Teague, J. W.
481bf04e-9cd4-4acf-8824-2a5f7cfc9dca
Collins, A.
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Morton, N. E.
c668e2be-074a-4a0a-a2ca-e8f51830ebb7
1 January 1996
Teague, J. W.
481bf04e-9cd4-4acf-8824-2a5f7cfc9dca
Collins, A.
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Morton, N. E.
c668e2be-074a-4a0a-a2ca-e8f51830ebb7
Teague, J. W., Collins, A. and Morton, N. E.
(1996)
Studies on locus content mapping.
Proceedings of the National Academy of Sciences of the United States of America, 93 (21), .
(doi:10.1073/pnas.93.21.11814).
Abstract
Locus content maps are derived from monosomic or disomic chromosomes broken by radiation, shearing, or other clastogen, the fragments being distributed among clones by dilution or incorporation into the cells of another species and scored for segregation of markers. Locus content maps provide evidence about radiosensitivity of chromosome regions, support for order, and approximate location. Omission of the most aberrant and least informative clones increases efficiency of localization. Correct analysis must allow for preferential retention of certain sequences, monosomy or polysomy of donor chromosomes, and error filtration. Combination of these refinements extracts substantially more information from fewer clones. Because of unmodeled peculiarities in the data, the best analysis does not recover the physical map but roughly localizes markers that may be monomorphic and therefore unsuitable for linkage mapping. As with linkage for polymorphic loci, distance in the composite map should be confirmed by physical methods
This record has no associated files available for download.
More information
Published date: 1 January 1996
Identifiers
Local EPrints ID: 470612
URI: http://eprints.soton.ac.uk/id/eprint/470612
ISSN: 0027-8424
PURE UUID: 6ea9e7f9-d16d-4388-b518-1570f5c30b44
Catalogue record
Date deposited: 14 Oct 2022 16:43
Last modified: 17 Mar 2024 02:37
Export record
Altmetrics
Contributors
Author:
J. W. Teague
Author:
N. E. Morton
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
