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A light-guiding urinary catheter for the inhibition of Proteus mirabilis biofilm formation

A light-guiding urinary catheter for the inhibition of Proteus mirabilis biofilm formation
A light-guiding urinary catheter for the inhibition of Proteus mirabilis biofilm formation
Catheter-associated urinary tract infection (CAUTI) is a leading cause of hospital-acquired infections worldwide causing debilitating illness for patients as well as a significant financial and treatment burden on health services. CAUTI is linked with the build-up of biofilms on catheter surfaces which act as a reservoir for infection. Additionally, urease-producing bacteria such as Gram-negative Proteus mirabilis (PM), can form crystalline biofilms which encrust catheter surfaces ultimately leading to blockages which require immediate removal of the catheter. Currently there are limited treatments available to prevent the formation of biofilms by PM as well as other urinary tract infection causing bacteria. A novel concept for a light-guiding urinary catheter is presented where a silicone elastomer waveguide incorporated along the length of the catheter is used to irradiate the catheter surfaces with antimicrobial blue light (405 nm) to prevent biofilm formation in situ. The prototype device is mass producible while also easy to fabricate in a lab setting for research studies. The inhibitory effect of blue light on PM biofilm formation over a range of irradiances is described for the first time showing an LD90 at 192–345 J/cm2 and total inhibition at 1,700 J/cm2 In vitro studies show that the light-guiding catheter (LGC) prototypes exhibit a 98% inhibition in PM biofilm formation inside the catheter lumen at an average estimated irradiance of 30–50 mW/cm2 (324–540 J/cm2 fluence) showing that the concept is highly effective, promising to be a powerful and economical antimicrobial approach to prevent catheter associated biofilm development and blockage.
1664-302X
Butement, Jonathan
581ce321-f1af-4a2f-870a-9d8d45133586
Noel, Daniel J.
75de38f4-0e98-4806-8c03-fc749c76c0f9
Bryant, Catherine A.
291101ce-79ed-4419-adea-322d3136138c
Wilks, Sandra A.
f1112b87-6aa3-43be-8f64-ec1728dbd8bd
Eason, Robert W.
2c4b791d-4d48-4e94-993d-f3199591e51a
Butement, Jonathan
581ce321-f1af-4a2f-870a-9d8d45133586
Noel, Daniel J.
75de38f4-0e98-4806-8c03-fc749c76c0f9
Bryant, Catherine A.
291101ce-79ed-4419-adea-322d3136138c
Wilks, Sandra A.
f1112b87-6aa3-43be-8f64-ec1728dbd8bd
Eason, Robert W.
2c4b791d-4d48-4e94-993d-f3199591e51a

Butement, Jonathan, Noel, Daniel J., Bryant, Catherine A., Wilks, Sandra A. and Eason, Robert W. (2022) A light-guiding urinary catheter for the inhibition of Proteus mirabilis biofilm formation. Frontiers in Microbiology. (doi:10.3389/fmicb.2022.995200).

Record type: Special issue

Abstract

Catheter-associated urinary tract infection (CAUTI) is a leading cause of hospital-acquired infections worldwide causing debilitating illness for patients as well as a significant financial and treatment burden on health services. CAUTI is linked with the build-up of biofilms on catheter surfaces which act as a reservoir for infection. Additionally, urease-producing bacteria such as Gram-negative Proteus mirabilis (PM), can form crystalline biofilms which encrust catheter surfaces ultimately leading to blockages which require immediate removal of the catheter. Currently there are limited treatments available to prevent the formation of biofilms by PM as well as other urinary tract infection causing bacteria. A novel concept for a light-guiding urinary catheter is presented where a silicone elastomer waveguide incorporated along the length of the catheter is used to irradiate the catheter surfaces with antimicrobial blue light (405 nm) to prevent biofilm formation in situ. The prototype device is mass producible while also easy to fabricate in a lab setting for research studies. The inhibitory effect of blue light on PM biofilm formation over a range of irradiances is described for the first time showing an LD90 at 192–345 J/cm2 and total inhibition at 1,700 J/cm2 In vitro studies show that the light-guiding catheter (LGC) prototypes exhibit a 98% inhibition in PM biofilm formation inside the catheter lumen at an average estimated irradiance of 30–50 mW/cm2 (324–540 J/cm2 fluence) showing that the concept is highly effective, promising to be a powerful and economical antimicrobial approach to prevent catheter associated biofilm development and blockage.

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Accepted/In Press date: 30 August 2022
Published date: 20 September 2022

Identifiers

Local EPrints ID: 470617
URI: http://eprints.soton.ac.uk/id/eprint/470617
ISSN: 1664-302X
PURE UUID: 96cc0c33-77c0-4aff-bb5c-db761a40af3b

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Date deposited: 14 Oct 2022 16:46
Last modified: 14 Oct 2022 16:47

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Contributors

Author: Jonathan Butement
Author: Daniel J. Noel
Author: Catherine A. Bryant
Author: Sandra A. Wilks
Author: Robert W. Eason

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