Enhancer SINEs link Pol III to Pol II transcription in neurons
Enhancer SINEs link Pol III to Pol II transcription in neurons
Spatiotemporal regulation of gene expression depends on the cooperation of multiple mechanisms, including the functional interaction of promoters with distally located enhancers. Here, we show that, in cortical neurons, a subset of short interspersed nuclear elements (SINEs) located in the proximity of activity-regulated genes bears features of enhancers. Enhancer SINEs (eSINEs) recruit the Pol III cofactor complex TFIIIC in a stimulus-dependent manner and are transcribed by Pol III in response to neuronal depolarization. Characterization of an eSINE located in proximity to the Fos gene (FosRSINE1) indicated that the FosRSINE1-encoded transcript interacts with Pol II at the Fos promoter and mediates Fos relocation to Pol II factories, providing an unprecedented molecular link between Pol III and Pol II transcription. Strikingly, knockdown of the FosRSINE1 transcript induces defects of both cortical radial migration in vivo and activity-dependent dendritogenesis in vitro, demonstrating that FosRSINE1 acts as a strong enhancer of Fos expression in diverse physiological contexts.
2879-2894
Policarpi, Cristina
1c9eb268-3724-4ba4-8562-952ab370d127
Crepaldi, Luca
ac91307e-5dcf-4bc8-badd-25dc4147427a
Brookes, Emily
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Nitarska, Justyna
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French, Sarah M.
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Coatti, Alessandro
00e18a05-212c-45ad-805f-a328fce1779a
Riccio, Antonella
44c9689b-90bb-4f46-9d8a-24680bdf3483
December 2017
Policarpi, Cristina
1c9eb268-3724-4ba4-8562-952ab370d127
Crepaldi, Luca
ac91307e-5dcf-4bc8-badd-25dc4147427a
Brookes, Emily
425dafc2-111b-4f6c-9336-f720c4ef8cac
Nitarska, Justyna
7cb2c560-2137-482b-88fe-f47a65621ea8
French, Sarah M.
ff919c6d-f0e7-4f91-816a-b469c528b21c
Coatti, Alessandro
00e18a05-212c-45ad-805f-a328fce1779a
Riccio, Antonella
44c9689b-90bb-4f46-9d8a-24680bdf3483
Policarpi, Cristina, Crepaldi, Luca, Brookes, Emily, Nitarska, Justyna, French, Sarah M., Coatti, Alessandro and Riccio, Antonella
(2017)
Enhancer SINEs link Pol III to Pol II transcription in neurons.
Cell Reports, 21 (10), .
(doi:10.1016/j.celrep.2017.11.019).
Abstract
Spatiotemporal regulation of gene expression depends on the cooperation of multiple mechanisms, including the functional interaction of promoters with distally located enhancers. Here, we show that, in cortical neurons, a subset of short interspersed nuclear elements (SINEs) located in the proximity of activity-regulated genes bears features of enhancers. Enhancer SINEs (eSINEs) recruit the Pol III cofactor complex TFIIIC in a stimulus-dependent manner and are transcribed by Pol III in response to neuronal depolarization. Characterization of an eSINE located in proximity to the Fos gene (FosRSINE1) indicated that the FosRSINE1-encoded transcript interacts with Pol II at the Fos promoter and mediates Fos relocation to Pol II factories, providing an unprecedented molecular link between Pol III and Pol II transcription. Strikingly, knockdown of the FosRSINE1 transcript induces defects of both cortical radial migration in vivo and activity-dependent dendritogenesis in vitro, demonstrating that FosRSINE1 acts as a strong enhancer of Fos expression in diverse physiological contexts.
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1-s2.0-S221112471731639X-main
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Accepted/In Press date: 2 November 2017
Published date: December 2017
Identifiers
Local EPrints ID: 471261
URI: http://eprints.soton.ac.uk/id/eprint/471261
ISSN: 2211-1247
PURE UUID: 934e23a1-4902-47e9-8459-6621b2350204
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Date deposited: 01 Nov 2022 17:44
Last modified: 17 Mar 2024 04:14
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Contributors
Author:
Cristina Policarpi
Author:
Luca Crepaldi
Author:
Emily Brookes
Author:
Justyna Nitarska
Author:
Sarah M. French
Author:
Alessandro Coatti
Author:
Antonella Riccio
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