READ ME File For 'Datasets associated with the publication IL-33 induces an antiviral signature in mast cells but enhances their permissiveness for human rhinovirus infection' Dataset DOI: 10.5258/SOTON/D2415 Date that the file was created: October, 2022 ------------------- GENERAL INFORMATION ------------------- ReadMe Author: Dr Emily Swindle, University of Southampton [0000-0003-3644-7747] Date of data collection: November 2017 - Sept 2022 Information about geographic location of data collection: University of Southampton, UK Related projects: Charlene Akoto 2017. The Susceptibility of Mast Cells for Rhinovirus Infection: Implications for Asthma Exacerbations (embargoed doctoral thesis, University of Southampton, UK) Chiara Banas 2022. Role of IL-33 on Human Mast Cell Responses to Rhinovirus Infection in Asthma (unpublished doctoral thesis, University of Southampton, UK) -------------------------- SHARING/ACCESS INFORMATION -------------------------- Licenses/restrictions placed on the data, or limitations of reuse: Recommended citation for the data: This dataset supports the publication: AUTHORS:Charlene Akoto, Anna Willis, Chiara Banas, Joseph A. Bell, Dean Bryant, Cornelia Blume, Donna E. Davies and Emily J. Swindle TITLE:IL-33 induces an antiviral signature in mast cells but enhances their permissiveness for human rhinovirus infection JOURNAL: Viruses PAPER DOI IF KNOWN: Links to other publicly accessible locations of the data: Links/relationships to ancillary or related data sets: -------------------- DATA & FILE OVERVIEW -------------------- This dataset contains: 4 Prism files detailed below (1) Akoto et al 2022_viruses_Main Figs.pzfx - contains the data used for Figures 3 -6 and Suppl Figures A3A-C and A4C (2) Akoto et al 2022_viruses_Fig 2D.pzfx - contains the data used for Figure 2D (3) Akoto et al 2022_Viruses_Suppl Fig A4AB_ICAM-1 and CDHR3_validation_avCt.pzfx - contains the data used for Suppl Figure A4A-B (4) Akoto et al 2022_Viruses_Suppl Fig A5 - contains the data used for Suppl Figure A5 Relationship between files, if important for context: N/A Additional related data collected that was not included in the current data package: Transcriptomic data used to compile Figures 1-2 are publically as a GEO Dataset (https://www.ncbi.nlm.nih.gov/gds). Specific details in main manuscript of paper If data was derived from another source, list source: N/A If there are there multiple versions of the dataset, list the file updated, when and why update was made: N/A -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: See methods section of publication Methods for processing the data: (1) Quantitative PCR data was collected via the BioRad iCycler CFX software on a local secure computer linked to the iCycler PCR machine. Data underwent quality control (QC) prior to exporting as .xlsx files for calculation of 2^ddCT and then transferred to Prism and stored as .pzfx files (2) ELISA protein data was generated via the Ascent multiskan software on a local secure computer linked to the Fluoroskan microplate reader. Data underwent QC prior to transfer to Prism and stored as .pzfx files (3) Flow cytometric data was generated via a FACSCaliber cell analyser and linked software on a local secure computer. Data underwent QC and analysed using FlowJo software (version 7.6.5) prior to transfer to Prism and stored as .pzfx files (4) TCID50 data was inputted and stored as .xlxs files Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: Graphpad Prism Version 9.4.1 Standards and calibration information, if appropriate: N/A Environmental/experimental conditions: See methods section of publication Describe any quality-assurance procedures performed on the data: People involved with sample collection, processing, analysis and/or submission: Charlene Akoto, Anna Willis, Chiara Banas, Joseph A. Bell, Dean Bryant, Cornelia Blume, Donna E. Davies and Emily J. Swindle -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Number of variables: See publication Number of cases/rows: N/A Variable list, defining any abbreviations, units of measure, codes or symbols used: See publication Missing data codes: N/A Specialized formats or other abbreviations used: N/A