A versatile, low cost light source module for multiphoton imaging
A versatile, low cost light source module for multiphoton imaging
Multiphoton imaging methods such as Coherent Raman Scattering (CRS) microscopy which also comprises Second Harmonic Generation (SHG) and Two Photon Excited Auto-Fluorescence (TPEAF) imaging (termed as multimodal Coherent Raman microscopy), have greatly facilitated the advancement of biomedical research due to their unique features. Multimodal CRS microscopy, is label free, chemically specific, inherently 'confocal' offering three independent contrast mechanisms which can be associated in a composite image comprising a wide range of chemical and structural information about the interrogated sample. The standard light source for multimodal CRS microscopy is a picosecond pumped Optical Parametric Oscillator (OPO) which has exhibited excellent performance but due to its associated high cost, maintenance, complexity and requirement of a dedicated optics laboratory, has hindered the wider adoption of multimodal CRS microscopy and especially its deployment in clinical applications. Here we present a novel, low cost Optical Parametric Amplifier (OPA) based on a MgO doped Periodically Poled Lithium Niobate (PPLN) crystal seeded by a continuous wave (CW) laser source and pumped by a picosecond laser at 1031nm, which removes any synchronisation requirements. We show that this OPA is a versatile light source module that can be tailored to the tunability and affordability requirements of the specific application. We demonstrate that it can be used either in association with an OPO or on its own as a light source for multimodal CRS microscopy and we show its performance by imaging a variety of standards and biological samples.
Multimodal microscopy, Multiphoton imaging, Optical parametric amplifier
Bourdakos, Konstantinos N.
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Xu, Duanyang
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Johnson, Peter B.
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Crisford, Anna
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Xu, Lin
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Liang, Sijing
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Price, Jonathan H.V.
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Richardson, David J.
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Bourdakos, Konstantinos N.
83f6fc3a-db12-476b-9a78-4aad8756f82f
Xu, Duanyang
2eeb11e3-d31e-4552-a925-49a3ffd7da20
Johnson, Peter B.
1afe5cab-d45a-4cfe-91d9-d212e03cf1f2
Crisford, Anna
135675e1-a172-4d93-989b-93d1efb022c3
Xu, Lin
b887cecd-d21e-49f4-9b45-6909a7369e84
Liang, Sijing
2fe0bfcc-99f0-46ee-8a2e-2a7857fbfb74
Price, Jonathan H.V.
fddcce17-291b-4d01-bd38-8fb0453abdc8
Richardson, David J.
ebfe1ff9-d0c2-4e52-b7ae-c1b13bccdef3
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Bourdakos, Konstantinos N., Xu, Duanyang, Johnson, Peter B., Crisford, Anna, Xu, Lin, Liang, Sijing, Price, Jonathan H.V., Richardson, David J. and Mahajan, Sumeet
(2021)
A versatile, low cost light source module for multiphoton imaging.
Mahajan, Sumeet and Reichelt, Stefanie
(eds.)
In Frontiers in Biophotonics and Imaging.
vol. 11879,
SPIE..
(doi:10.1117/12.2601570).
Record type:
Conference or Workshop Item
(Paper)
Abstract
Multiphoton imaging methods such as Coherent Raman Scattering (CRS) microscopy which also comprises Second Harmonic Generation (SHG) and Two Photon Excited Auto-Fluorescence (TPEAF) imaging (termed as multimodal Coherent Raman microscopy), have greatly facilitated the advancement of biomedical research due to their unique features. Multimodal CRS microscopy, is label free, chemically specific, inherently 'confocal' offering three independent contrast mechanisms which can be associated in a composite image comprising a wide range of chemical and structural information about the interrogated sample. The standard light source for multimodal CRS microscopy is a picosecond pumped Optical Parametric Oscillator (OPO) which has exhibited excellent performance but due to its associated high cost, maintenance, complexity and requirement of a dedicated optics laboratory, has hindered the wider adoption of multimodal CRS microscopy and especially its deployment in clinical applications. Here we present a novel, low cost Optical Parametric Amplifier (OPA) based on a MgO doped Periodically Poled Lithium Niobate (PPLN) crystal seeded by a continuous wave (CW) laser source and pumped by a picosecond laser at 1031nm, which removes any synchronisation requirements. We show that this OPA is a versatile light source module that can be tailored to the tunability and affordability requirements of the specific application. We demonstrate that it can be used either in association with an OPO or on its own as a light source for multimodal CRS microscopy and we show its performance by imaging a variety of standards and biological samples.
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More information
e-pub ahead of print date: 6 October 2021
Additional Information:
Funding Information: EPSRC Transformative Healthcare 2050 grant (EP/T020997/1)
Venue - Dates:
Frontiers in Biophotonics and Imaging 2021, , Glasgow, United Kingdom, 2021-09-28 - 2021-09-30
Keywords:
Multimodal microscopy, Multiphoton imaging, Optical parametric amplifier
Identifiers
Local EPrints ID: 471354
URI: http://eprints.soton.ac.uk/id/eprint/471354
ISSN: 0277-786X
PURE UUID: 3165c26c-7f9e-473c-bc13-85615986da60
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Date deposited: 03 Nov 2022 18:00
Last modified: 18 Mar 2024 03:32
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Contributors
Author:
Konstantinos N. Bourdakos
Author:
Duanyang Xu
Author:
Peter B. Johnson
Author:
Jonathan H.V. Price
Editor:
Sumeet Mahajan
Editor:
Stefanie Reichelt
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