Monitoring aerobic marine bacterial biofilms on gold electrode surfaces and the influence of nitric oxide attachment control
Monitoring aerobic marine bacterial biofilms on gold electrode surfaces and the influence of nitric oxide attachment control
Detection of aerobic marine bacterial biofilms using electrochemical impedance spectroscopy monitored the interfacial re-sponse of Pseudoalteromonas sp. NCIMB 2021 attachment and growth to identify characteristic events on a 0.2 mm diam-eter gold electrode surface. Uniquely, the applicability of surface charge density has been proven to be valuable in determin-ing biofilm attachment and cell enumeration over a 72 h duration on a gold surface within a modified continuous culture flow cell (a controlled low laminar flow regime with a Reynolds number 1). In addition, biofilm dispersal has been evaluat-ed using 500 nM sodium nitroprusside, nitric oxide donor (nitric oxide is important for the regulation of several diverse bio-logical processes). Ex situ confocal microscopy studies were performed to confirm biofilm coverage and morphology, plus the determination and quantification of the nitric oxide biofilm dispersal effects. Overall, the capability of the sensor to elec-trochemically detect the presence of initial bacterial biofilm formation and extent has been established and shown to have potential for real-time biofilm monitoring.
12323-12332
Werwinski, Stephane
f38f49f0-ebff-4bb5-9fe2-f5da28695bbb
Wharton, Julian
965a38fd-d2bc-4a19-a08c-2d4e036aa96b
Nie, Mengyan
a8613738-d74a-40fb-8881-08069fb0a34b
Stokes, Keith
5fb4e7f7-2f7e-4e6e-a045-6d7690626695
31 August 2022
Werwinski, Stephane
f38f49f0-ebff-4bb5-9fe2-f5da28695bbb
Wharton, Julian
965a38fd-d2bc-4a19-a08c-2d4e036aa96b
Nie, Mengyan
a8613738-d74a-40fb-8881-08069fb0a34b
Stokes, Keith
5fb4e7f7-2f7e-4e6e-a045-6d7690626695
Werwinski, Stephane, Wharton, Julian, Nie, Mengyan and Stokes, Keith
(2022)
Monitoring aerobic marine bacterial biofilms on gold electrode surfaces and the influence of nitric oxide attachment control.
Analytical Chemistry, 94 (36), .
(doi:10.1021/acs.analchem.2c00934).
Abstract
Detection of aerobic marine bacterial biofilms using electrochemical impedance spectroscopy monitored the interfacial re-sponse of Pseudoalteromonas sp. NCIMB 2021 attachment and growth to identify characteristic events on a 0.2 mm diam-eter gold electrode surface. Uniquely, the applicability of surface charge density has been proven to be valuable in determin-ing biofilm attachment and cell enumeration over a 72 h duration on a gold surface within a modified continuous culture flow cell (a controlled low laminar flow regime with a Reynolds number 1). In addition, biofilm dispersal has been evaluat-ed using 500 nM sodium nitroprusside, nitric oxide donor (nitric oxide is important for the regulation of several diverse bio-logical processes). Ex situ confocal microscopy studies were performed to confirm biofilm coverage and morphology, plus the determination and quantification of the nitric oxide biofilm dispersal effects. Overall, the capability of the sensor to elec-trochemically detect the presence of initial bacterial biofilm formation and extent has been established and shown to have potential for real-time biofilm monitoring.
Text
acs.analchem.2c00934
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Accepted/In Press date: 14 August 2022
Published date: 31 August 2022
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Funding Information:
The authors also like to thank Dr H. Schuppe and Prof J.S. Webb from the Institute for Life Sciences (IfLS) at the University of Southampton and also Dr J.R. Gittins from the National Oceanography Centre (NOC) for technical support and expertise on the confocal microscopy technique and the bacteria culture protocol.
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© 2022 The Authors. Published by American Chemical Society.
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Local EPrints ID: 471552
URI: http://eprints.soton.ac.uk/id/eprint/471552
ISSN: 0003-2700
PURE UUID: d570f94b-7184-4501-ae3f-7851a5a0f650
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Date deposited: 10 Nov 2022 18:02
Last modified: 17 Mar 2024 02:46
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Author:
Stephane Werwinski
Author:
Mengyan Nie
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