(2021) High-speed video microscopy analysis of cilia before and after airway cell culture. Zenodo doi:10.5281/zenodo.5228294 [Dataset]
Abstract
High-speed video microscopy analysis (HSVA) is a diagnostic tool used within the UK Primary Ciliary Dyskinesia (PCD) Service to access airway ciliary function on nasal brushing biopsies. The Southampton PCD group is based at the University of Southampton, Faculty of Medicine and the University Hospital Southampton NHS Foundation Trust and is led by Professor Jane Lucas. We also use air-liquid interface (ALI) culture to differentiate airway epithelial cells to regrow healthy cilia to repeat standard PCD tests (including HSVA, immunofluoresence labelling of cilia proteins, transmission electron microscopy and functional genomics) and provide PCD research samples, which also allow us to develop new diagnostic approaches. ALI-culture can restore normal ciliary movement when secondary damage (due to infection or poor cell health) temporarily causes of abnormal cilia movement or a lack of cilia. ALI-culture can also re-confirm when ciliary defects and abnormal ciliary function are permanent and cause by inherited PCD (a ciliopathy).
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