READ ME File For ''High-power, high-efficiency, all-fiberized-laser-pumped, 260-nm, deep-UV laser for bacterial deactivation'

Dataset DOI: 10.5258/SOTON/D1981


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GENERAL INFORMATION
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ReadMe Author: Qiang Fu, University of Southampton 

Date of data collection: 01/11/2019



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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse:CC-BY


This dataset supports the publication:
AUTHORS:Qiang Fu, Niall Hanrahan, Lin Xu, Simon Lane, Di Lin, Yongmin JUng, Sumeet Mahajan, and David J. Richardson
TITLE:High-power, high-efficiency, all-fiberized-laser-pumped, 260-nm, deep-UV laser for bacterial deactivation
JOURNAL:Optics Express
PAPER DOI IF KNOWN:https://doi.org/10.1364/OE.441248


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DATA & FILE OVERVIEW
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This dataset contains experimental data for the paper

The figures are as follows:

Fig. 2  Fig. 2. (a) Normalized spectra from the SBS monitor port at 585 ps, 1 ns, and 2 ns 1040-nm pulse duration and with different peak powers (0.01-nm resolution). (b) Output spectra of the YDF MOPA at a pulse duration of 585 ps and different output peak powers (0.01-nm resolution). Inset: Output spectra of the YDF MOPA over a large wavelength scale (1-nm resolution).
Fig. 3  Output power characteristics of the YDF MOPA system. Inset: temporal profile of the 1040 nm pulses at a pulse duration of 585 ps.
Fig. 5  Output power and conversion efficiency of SHG at a repetition rate of (a) 800 kHz and (b) 1.6 MHz.
Fig. 6  (a) Temporal profile for the SHG pulse. (b) Beam quality measurements for the SHG beam. Inset: SHG spectra measured at a resolution of 0.05 nm.
Fig. 7  (a) Output power characteristics for different 520 nm pump conditions. (b) Output power characteristics and conversion efficiencies of FHG at a repetition rate of 1.6 MHz and a beam waist of 30 �m.
Fig. 8  (a) Power stability measurement of DUV laser at 5.8 W. Inset: DUV laser spectra with ~1 nm resolution. (b) DUV beam quality measurement at an output power of 5.8 W.
Fig. 9. Survival assay of live E. Coli bacteria relative to experimental control with changing DUV dose on exposure to the pulsed DUV laser system and an LED source. The y-axis shows the proportion of bacteria remaining in samples that were exposed to DUV doses compared to unexposed samples. 
Fig. S2. Standards and controls supporting Bacterial growth data and calibration curve. (a) Absorbance time series of experimental blanks (no bacteria) demonstrating no bacterial growth in stock LB media. (b) Absorbance times series of bacteria dilution standards from 1/61 to 1/68 used for creating a standard curve. Growth profiles show the mean and standard deviation, where the shaded areas above and below the curves represent one standard deviation across measurements performed in duplicate. (c) Calibration curve for bacterial growth across 7 orders of magnitude, relating the time at which values cross the absorbance threshold (0.15) to the bacteria dilution factor in the standard samples. (d) Growth curves from experimental samples following UV irradiation showing the mean and standard deviation, where shaded areas above and below the curves represent one standard deviation across measurements performed in triplicate
 

Related projects:
Engineering and Physical Sciences Research Council (EPSRC) AirGuide Photonics Programme Grant (EP/P030181/1) InLightenUs Transformative Healthcare 2050 project (EP/T020997/1). Funding for this work was also provided by an EPSRC Impact Acceleration 2020/21 award and by the Institute for Life Sciences at the University of Southampton. 


Date that the file was created: 11,2022