A comparison of non-integrating reprogramming methods

Schlaeger, Thorsten M., Daheron, Laurence, Brickler, Thomas R., Entwisle, Samuel, Chan, Karrie, Cianci, Amelia, DeVine, Alexander, Ettenger, Andrew, Brookes, Emily and et al., (2015) A comparison of non-integrating reprogramming methods. Nature Biotechnology, 33, 58-63. (doi:10.1038/nbt.3070).

Abstract

Human induced pluripotent stem cells (hiPSCs1,2,3) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV)4, episomal (Epi)5 and mRNA transfection mRNA6 methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.

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Contributors

Author: Emily Brookes

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