Ontogenetic changes in cortical bone vascular microstructure in the domestic duck (Anas platyrhynchos) and ring-necked pheasant (Phasianus colchicus)
Ontogenetic changes in cortical bone vascular microstructure in the domestic duck (Anas platyrhynchos) and ring-necked pheasant (Phasianus colchicus)
Age-related changes in bone microstructure can inform our understanding the biology of both extant and fossil birds, but to date, histological work in birds, and particularly work using high-resolution 3D imaging, has largely been restricted to limited growth stages. We used minimally destructive synchrotron radiation-based X-ray computed tomography to visualise and measure key morphological and histological traits in 3D across development in the domestic duck and ring-necked pheasant. We use these measurements to build on the database of key reference material for interpreting bone histology. We found that growth patterns differed between the two species, with the ducks showing rapid growth in their lower limbs and early lower limb maturation, while pheasants grew more slowly, reflecting their later age at maturity. In the pheasant, both walking and flight occur early and their upper and lower limbs grew at similar rates. In the duck, flight and wing development are delayed until the bird is almost at full body mass. Through juvenile development, the second moment of area for the duck wing was low but increased rapidly towards the age of flight, at which point it became significantly greater than that of the lower limb, or the pheasant. On a microstructural level, both cortical porosity and canal diameter were related to cortical bone deposition rate. In terms of orientation, vascular canals in the bone cortex were more laminar in the humerus and femur compared with the tibiotarsus, and laminarity increased through juvenile development in the humerus, but not the tibiotarsus, possibly reflecting torsional vs compressive loading. These age-related changes in cortical bone vascular microstructure of the domestic duck and pheasant will help understanding the biology of both extant and fossil birds, including age estimation, growth rate and growth patterns, and limb function.
avian, bird, bone, development, histology, microstructure, palaeontology, vasculature
1371-1386
Williams, Katherine
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Gostling, Neil J.
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Oreffo, Richard
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Schneider, Philipp
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December 2022
Williams, Katherine
a8e31587-75d2-4bb9-b45b-259b3f3b22aa
Gostling, Neil J.
4840aa40-cb6c-4112-a0b9-694a869523fc
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Williams, Katherine, Gostling, Neil J., Oreffo, Richard and Schneider, Philipp
(2022)
Ontogenetic changes in cortical bone vascular microstructure in the domestic duck (Anas platyrhynchos) and ring-necked pheasant (Phasianus colchicus).
Journal of Anatomy, 241 (6), .
(doi:10.1111/joa.13741).
Abstract
Age-related changes in bone microstructure can inform our understanding the biology of both extant and fossil birds, but to date, histological work in birds, and particularly work using high-resolution 3D imaging, has largely been restricted to limited growth stages. We used minimally destructive synchrotron radiation-based X-ray computed tomography to visualise and measure key morphological and histological traits in 3D across development in the domestic duck and ring-necked pheasant. We use these measurements to build on the database of key reference material for interpreting bone histology. We found that growth patterns differed between the two species, with the ducks showing rapid growth in their lower limbs and early lower limb maturation, while pheasants grew more slowly, reflecting their later age at maturity. In the pheasant, both walking and flight occur early and their upper and lower limbs grew at similar rates. In the duck, flight and wing development are delayed until the bird is almost at full body mass. Through juvenile development, the second moment of area for the duck wing was low but increased rapidly towards the age of flight, at which point it became significantly greater than that of the lower limb, or the pheasant. On a microstructural level, both cortical porosity and canal diameter were related to cortical bone deposition rate. In terms of orientation, vascular canals in the bone cortex were more laminar in the humerus and femur compared with the tibiotarsus, and laminarity increased through juvenile development in the humerus, but not the tibiotarsus, possibly reflecting torsional vs compressive loading. These age-related changes in cortical bone vascular microstructure of the domestic duck and pheasant will help understanding the biology of both extant and fossil birds, including age estimation, growth rate and growth patterns, and limb function.
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Journal of Anatomy - 2022 - Williams - Ontogenetic changes in cortical bone vascular microstructure in the domestic duck
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Accepted/In Press date: 28 July 2022
e-pub ahead of print date: 24 August 2022
Published date: December 2022
Additional Information:
Funding Information:
This work was funded by the Institute for Life Sciences at the University of Southampton and the Faculty of Engineering and Physical Sciences at the University of Southampton through its Engineering and Physical Sciences Research Council (EPSRC) Doctoral Training Programme support. We acknowledge the Paul Scherrer Institut, Villigen, Switzerland for provision of synchrotron radiation beamtime at TOMCAT beamline of the Swiss Light Source and would like to thank Pablo Villanueva‐Perez and Goran Lovric for their assistance. We acknowledge Diamond Light Source for beamtime at beamline I13‐2 and would like to thank Andrew Bodey and Shashidhara Marathe for their assistance. We would also like to thank Christian Laurent, Elis Newham, Juan Núñez, Colin Palmer, Hannah Cheales, Roeland de Kat, Richard Cook, Aileen O'Brien, Alisha Sharma, Harry Rossides, Roxanna Ramnarine Sanchez and Erik Meilak for their assistance during data acquisition. The authors acknowledge the μ‐VIS X‐ray Imaging Centre at the University of Southampton for provision of computing facilities, supported by EPSRC grant EP‐H01506X. We would also like to thank Siul Ruiz for proofreading this manuscript and discussions regarding bone mechanics.
Funding Information:
This work was funded by the Institute for Life Sciences at the University of Southampton and the Faculty of Engineering and Physical Sciences at the University of Southampton through its Engineering and Physical Sciences Research Council (EPSRC) Doctoral Training Programme support. We acknowledge the Paul Scherrer Institut, Villigen, Switzerland for provision of synchrotron radiation beamtime at TOMCAT beamline of the Swiss Light Source and would like to thank Pablo Villanueva-Perez and Goran Lovric for their assistance. We acknowledge Diamond Light Source for beamtime at beamline I13-2 and would like to thank Andrew Bodey and Shashidhara Marathe for their assistance. We would also like to thank Christian Laurent, Elis Newham, Juan Núñez, Colin Palmer, Hannah Cheales, Roeland de Kat, Richard Cook, Aileen O'Brien, Alisha Sharma, Harry Rossides, Roxanna Ramnarine Sanchez and Erik Meilak for their assistance during data acquisition. The authors acknowledge the μ-VIS X-ray Imaging Centre at the University of Southampton for provision of computing facilities, supported by EPSRC grant EP-H01506X. We would also like to thank Siul Ruiz for proofreading this manuscript and discussions regarding bone mechanics.
Publisher Copyright:
© 2022 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.
Keywords:
avian, bird, bone, development, histology, microstructure, palaeontology, vasculature
Identifiers
Local EPrints ID: 472322
URI: http://eprints.soton.ac.uk/id/eprint/472322
ISSN: 0021-8782
PURE UUID: 07996551-58a3-4cc1-a3b4-43dfb2645901
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Date deposited: 01 Dec 2022 17:39
Last modified: 17 Mar 2024 03:34
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Author:
Katherine Williams
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