Myo-inositol moderates glucose-induced effects on human placental 13C-arachidonic acid metabolism
Myo-inositol moderates glucose-induced effects on human placental 13C-arachidonic acid metabolism
Maternal hyperglycemia is associated with disrupted transplacental arachidonic acid (AA) supply and eicosanoid synthesis, which contribute to adverse pregnancy outcomes. Since placental inositol is lowered with increasing glycemia, and since myo-inositol appears a promising intervention for gestational diabetes, we hypothesized that myo-inositol might rectify glucose-induced perturbations in placental AA metabolism. Term placental explants (n = 19) from women who underwent a mid-gestation oral glucose-tolerance-test were cultured with
13C-AA for 48 h in media containing glucose (5, 10 or 17 mM) and myo-inositol (0.3 or 60 µM). Newly synthesized
13C-AA-lipids were quantified by liquid-chromatography-mass-spectrometry. Increasing maternal fasting glycemia was associated with decreased proportions of
13C-AA-phosphatidyl-ethanolamines (PE, PE-P), but increased proportions of
13C-AA-triacylglycerides (TGs) relative to total placental
13C-AA lipids. This suggests altered placental AA compartmentalization towards storage and away from pools utilized for eicosanoid production and fetal AA supply. Compared to controls (5 mM glucose), 10 mM glucose treatment decreased the amount of four
13C-AA-phospholipids and eleven
13C-AA-TGs, whilst 17 mM glucose increased
13C-AA-PC-40:8 and
13C-AA-LPC. Glucose-induced alterations in all
13C-AA lipids (except PE-P-38:4) were attenuated by concurrent 60 µM myo-inositol treatment. Myo-inositol therefore rectifies some glucose-induced effects, but further studies are required to determine if maternal myo-inositol supplementation could reduce AA-associated pregnancy complications.
LCMS, diabetes, lipid, metabolism, pregnancy
Watkins, Oliver C.
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Cracknell-Hazra, Victoria K.B.
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Pillai, Reshma Appukuttan
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Selvam, Preben
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Yong, Hannah E.J.
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Sharma, Neha
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Patmanathan, Sathya Narayanan
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Cazenave-Gassiot, Amaury
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Bendt, Anne K.
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Godfrey, Keith
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Lewis, Rohan
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Wenk, Markus R.
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Chan, Shiao-Yng
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26 September 2022
Watkins, Oliver C.
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Cracknell-Hazra, Victoria K.B.
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Pillai, Reshma Appukuttan
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Selvam, Preben
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Yong, Hannah E.J.
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Sharma, Neha
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Patmanathan, Sathya Narayanan
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Cazenave-Gassiot, Amaury
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Bendt, Anne K.
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Godfrey, Keith
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Lewis, Rohan
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Wenk, Markus R.
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Chan, Shiao-Yng
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Watkins, Oliver C., Cracknell-Hazra, Victoria K.B., Pillai, Reshma Appukuttan, Selvam, Preben, Yong, Hannah E.J., Sharma, Neha, Patmanathan, Sathya Narayanan, Cazenave-Gassiot, Amaury, Bendt, Anne K., Godfrey, Keith, Lewis, Rohan, Wenk, Markus R. and Chan, Shiao-Yng
(2022)
Myo-inositol moderates glucose-induced effects on human placental 13C-arachidonic acid metabolism.
Nutrients, 14 (19), [3988].
(doi:10.3390/nu14193988).
Abstract
Maternal hyperglycemia is associated with disrupted transplacental arachidonic acid (AA) supply and eicosanoid synthesis, which contribute to adverse pregnancy outcomes. Since placental inositol is lowered with increasing glycemia, and since myo-inositol appears a promising intervention for gestational diabetes, we hypothesized that myo-inositol might rectify glucose-induced perturbations in placental AA metabolism. Term placental explants (n = 19) from women who underwent a mid-gestation oral glucose-tolerance-test were cultured with
13C-AA for 48 h in media containing glucose (5, 10 or 17 mM) and myo-inositol (0.3 or 60 µM). Newly synthesized
13C-AA-lipids were quantified by liquid-chromatography-mass-spectrometry. Increasing maternal fasting glycemia was associated with decreased proportions of
13C-AA-phosphatidyl-ethanolamines (PE, PE-P), but increased proportions of
13C-AA-triacylglycerides (TGs) relative to total placental
13C-AA lipids. This suggests altered placental AA compartmentalization towards storage and away from pools utilized for eicosanoid production and fetal AA supply. Compared to controls (5 mM glucose), 10 mM glucose treatment decreased the amount of four
13C-AA-phospholipids and eleven
13C-AA-TGs, whilst 17 mM glucose increased
13C-AA-PC-40:8 and
13C-AA-LPC. Glucose-induced alterations in all
13C-AA lipids (except PE-P-38:4) were attenuated by concurrent 60 µM myo-inositol treatment. Myo-inositol therefore rectifies some glucose-induced effects, but further studies are required to determine if maternal myo-inositol supplementation could reduce AA-associated pregnancy complications.
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nutrients-14-03988
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Accepted/In Press date: 22 September 2022
Published date: 26 September 2022
Keywords:
LCMS, diabetes, lipid, metabolism, pregnancy
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Local EPrints ID: 472335
URI: http://eprints.soton.ac.uk/id/eprint/472335
ISSN: 2072-6643
PURE UUID: 9bc04c6a-a0d3-453a-9a60-0b7d990ae840
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Date deposited: 01 Dec 2022 17:45
Last modified: 17 Mar 2024 02:53
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Contributors
Author:
Oliver C. Watkins
Author:
Victoria K.B. Cracknell-Hazra
Author:
Reshma Appukuttan Pillai
Author:
Preben Selvam
Author:
Hannah E.J. Yong
Author:
Neha Sharma
Author:
Sathya Narayanan Patmanathan
Author:
Amaury Cazenave-Gassiot
Author:
Anne K. Bendt
Author:
Markus R. Wenk
Author:
Shiao-Yng Chan
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