READ ME File For 'Dataset for: A proof‐of‐concept study of the removal of early and late phase biofilm from skin wound models using a liquid acoustic stream'

Dataset DOI: https://doi.org/10.5258/SOTON/D2193 

Date that the file was created: April 2022

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GENERAL INFORMATION
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ReadMe Author: Thomas James Secker, University of Southampton [https://orcid.org/0000-0001-7168-8592]

Date of data collection: 2016 - 2018


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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse: CC BY-NC

Recommended citation for the data:

This dataset supports the publication: A proof‐of‐concept study of the removal of early and late phase biofilm from skin wound models using a liquid acoustic stream
AUTHORS:Thomas J Secker, Christopher C Harling, Chloe Hand, Davd Voegeli, Charles W Keevil, Timothy G Leighton. 
TITLE:A proof‐of‐concept study of the removal of early and late phase biofilm from skin wound models using a liquid acoustic stream
JOURNAL:International Wound Journal 
PAPER DOI: 10.1111/iwj.13818


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DATA & FILE OVERVIEW
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This dataset contains:The raw data of the image analysis of the percentage coverage measurements in figures 3 and 4, and the measurements of re-epithelialisation in figures 6 and 7

[IWJ 10.1111_IWJ.13818 Total data.pdf]

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METHODOLOGICAL INFORMATION
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Figures 3 and 4 - ImageJ was used to analyse the percentage coverage of GFP signal corresponding to the bioburden of bacterial contamination of the wound models. 

Figures 6 and 7 - ImageJ was used to measure the length of re-epithelialisation in the treated and non treated EFT wounds following H&E and CK14 IHC staining. The measurements where controlled via the use of a sensitive graticule standard.