READ ME File For 'Dataset in support of the paper "Similarities between the biochemical composition of jellyfish body and mucus'

Dataset DOI: 10.5258/SOTON/D2476

Date that the file was created: January, 2022

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GENERAL INFORMATION
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ReadMe Author: Nathan Hubot, University of Southampton [orcid.org/0000-0001-6917-2255]

Date of data collection: 2019 - 2021

Information about geographic location of data collection: 

Horsea Lake (UK), Walvis Bay (Namibia), London Aquarium (UK), Port Elizabeth (South Africa) and the Isle of Portland (UK)

Related projects:
Researcher Training Support Grant (RTSG number: 517191102)

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SHARING/ACCESS INFORMATION
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Licenses/restrictions placed on the data, or limitations of reuse:
CC-BY

Recommended citation for the data:
Hubot, N., S. L. C. Giering, and C. H. Lucas. 2022. Similarities between the biochemical composition of jellyfish body and mucus. Journal of Plankton Research 44: 337–344. doi:10.1093/plankt/fbab091


This dataset supports the publication:
AUTHORS: Hubot, N., S. L. C. Giering, and C. H. Lucas
TITLE: Similarities between the biochemical composition of jellyfish body and mucus
JOURNAL: Journal of Plankton Research 
PAPER DOI IF KNOWN: doi:10.1093/plankt/fbab091

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DATA & FILE OVERVIEW
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This dataset contains:

Hubot_et_al_2022_data.csv

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METHODOLOGICAL INFORMATION
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Briefly: Total lipids were extracted using a single-step extraction method based on the chloroform–methanol solvent system following the protocol by Axelsson and Gentili (2014; see details in Supplementary Information (SI)). Total proteins were measured using a modification of the Lowry assay by Gerhardt et al. (1994; see details in SI). Total carbohydrates were measured following the protocol by DuBois et al. (1956, see details in SI). The carbon and nitrogen content of the samples were measured using a CHNS Elemental Analyzer (Elementar Vario Micro Cube, see details in SI). Ash-free dry weight (AFDW, i.e. the organic portion of the dry weight) was calculated by measuring the ash weight (AW) following combustion at 400°C in a muffle furnace using an ultra-microbalance (Sartorius SE2, readability: 0.1 μg; see details in SI) and subtracting this value from the dry weight (DW; AFDW = DW – AW). All samples were measured in triplicate.

For more details, see publication: Hubot, N., S. L. C. Giering, and C. H. Lucas. 2022. Similarities between the biochemical composition of jellyfish body and mucus. Journal of Plankton Research 44: 337–344. doi:10.1093/plankt/fbab091

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DATA-SPECIFIC INFORMATION <Create sections for each datafile or set, as appropriate>
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Number of variables: 11

Number of cases/rows: 154

Variable list:

sample (sample number)
Species (Species name)
Replicate (replicate number)
Tissue (Type of tissue; mucus or body)
Mol_type (Type of macromolecule; protein, lipid or carbohydrate)
Mol_conc (macromolecule concentration expressed in %DW; percentage of dry weight)
Inorg (proportion of inorganic material expressed in %DW; percentage of dry weight)
OM (proportion of organic material expressed in %DW; percentage of dry weight)
AFDW (Ash-free dry weight expressed in %DW; percentage of dry weight)
N% (nitrogen content expressed in percentage of total mass)
C% (carbon content expressed in percentage of total mass)