Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification
Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification
Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present orthogonal strategies to produce densely modified RCA products and DNFs. We provide methods to selectively modify the DNA component and/or the protein cargo of these materials, thereby greatly expanding the range of chemical functionalities available to these systems. We have used our methodology to construct DNFs bearing multiple surface aptamers and peptides capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating their potential for diagnostic and therapeutic applications.
9042–9052
Baker, Ysobel
4fceec1f-89ed-4a32-a753-8967daf6763a
Yuan, Liyiwen
5920c907-e385-4f8f-b9d1-c18c1c276e51
Chen, Jinfeng
1be70334-2c20-485f-8b22-467053f130cb
Belle, Roman
fad720b1-582e-44ff-960a-a614ac6b3d77
Carlisle, Robert
5fb8f722-76e1-468f-b8bc-62f81afad30c
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
17 August 2021
Baker, Ysobel
4fceec1f-89ed-4a32-a753-8967daf6763a
Yuan, Liyiwen
5920c907-e385-4f8f-b9d1-c18c1c276e51
Chen, Jinfeng
1be70334-2c20-485f-8b22-467053f130cb
Belle, Roman
fad720b1-582e-44ff-960a-a614ac6b3d77
Carlisle, Robert
5fb8f722-76e1-468f-b8bc-62f81afad30c
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Baker, Ysobel, Yuan, Liyiwen, Chen, Jinfeng, Belle, Roman, Carlisle, Robert, El-Sagheer, Afaf and Brown, Tom
(2021)
Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification.
Nucleic Acids Research, 49 (16), .
(doi:10.1093/nar/gkab720).
Abstract
Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present orthogonal strategies to produce densely modified RCA products and DNFs. We provide methods to selectively modify the DNA component and/or the protein cargo of these materials, thereby greatly expanding the range of chemical functionalities available to these systems. We have used our methodology to construct DNFs bearing multiple surface aptamers and peptides capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating their potential for diagnostic and therapeutic applications.
Text
gkab720
- Version of Record
Text
gkab720
- Version of Record
More information
Accepted/In Press date: 8 August 2021
Published date: 17 August 2021
Identifiers
Local EPrints ID: 472797
URI: http://eprints.soton.ac.uk/id/eprint/472797
ISSN: 0305-1048
PURE UUID: 86baed3c-eb3b-4323-bf5e-a5767d7b1058
Catalogue record
Date deposited: 19 Dec 2022 17:41
Last modified: 17 Mar 2024 04:17
Export record
Altmetrics
Contributors
Author:
Ysobel Baker
Author:
Liyiwen Yuan
Author:
Jinfeng Chen
Author:
Roman Belle
Author:
Robert Carlisle
Author:
Afaf El-Sagheer
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics