READ ME File For ‘Dataset for PhD Thesis’

Dataset DOI:  https://doi.org/10.5258/SOTON/D2205

ReadMe Author: Joao Fernandes, University of Southampton ORCID ID https://orcid.org/0000-0002-4504-8249

This dataset supports the thesis entitled “Development of an organ on chip microfluidic platform with real-time viability sensing capabilities”

AWARDED BY: University of Southampton
DATE OF AWARD: 2022

DESCRIPTION OF THE DATA
This data set includes excel files, text files, originGraph files and images related to data collected for Chapter 3, in which the electrical circuit model was tested with conductivity measurements and cell growth data, Chapter 4, which related to cell growth of Caco-2 or 16HBE14o- cells in the platform, and Chapter 5, in which a droplet generator platform was fabricated to assess analyte production over time.

Experiments:
- Cell media conductivity measured in microfluidic chips over time and data modelling.
- Measurement of PMMA disk placed in apical compartment of microfluidic chip, mimicking a change in resistance similar to that of a forming cell barrier.
- Measurement of 16HBE14o- epithelial barrier formation in the platform.
- Measurement of Caco-2 epithelial barrier formation in the platform.
- Flow-rate of mineral oil and water through the developed device.
- Droplet formation using a single-channel device (n=3).
- Droplet formation using a parallel 8-channel device (n=1).
- FITC diffusion through the porous support in the microfluidic chip.
- FITC-Dextran diffusion thought the porous support and a formed epithelial cell barrier.
- 16HBE14o- cells IL-8 production following apical stimulation with a viral mimetic.

The data was collected using a bespoke impedance analyser (Joel Bowring) alongside a script to covert the impedance data into a comparable ohms.cm2 format, a voltohmmeter and a confocal microscope. 

This dataset contains:
The corresponding data sets (excel, OPJ, txt) and images for the following figures and tables in Chapter 3: Table 3.1 and 3.2; Figure 3.14; 3.16 to 3.19. 

The corresponding data sets (excel, OPJ, txt) and images for the following figures in Chapter 4: Figure 4.9 to 4.13.

The corresponding data sets (excel, OPJ, txt) and images for the following figures in Chapter 5: Figure 5.8; 5.10; 5.12; 5.13 to 5.16.

Date of data collection: Sept 2017 to October 2021

Related projects/Funders:
J.R.C.C Fernandes, Development of an organ on a chip microfluidic platform with real-time viability sensing capabilities. University of Southampton, Southampton, UK. 

The vesicle work was funded by EV Foundry (Grant Agreement Number: 801367)

Related publication:
Published
J. Fernandes, N.Karra, J. Bowring, R. Reale, J. James, C. Blume, T.J. Pell, W.C. Rowan, D.E. Davies, E.J. Swindle, H. Morgan, “Real-Time Monitoring of Epithelial Barrier Function By Impedance Spectroscopy in a Microfluidic Platform” Lab Chip, 2022

In Preparation
J. Fernandes, N.Karra, E.J. Swindle, H. Morgan, “Droplet Fluidics for time-dependent analysis of barrier permeability in an epithelial barrier on chip system” Lab Chip, 2022

Date that the file was created: June, 2022