Microglia colonize the developing brain by clonal expansion of highly proliferative progenitors, following allometric scaling

Accepted/In Press date: 6 April 2023

e-pub ahead of print date: 25 April 2023

Published date: 30 May 2023

Additional Information: Funding Information: We thank the Southampton Flow Cytometry Facility and the Imaging Unit for technical advice and the Biomedical Research Facility for assistance with animal breeding and maintenance. We thank Georgina Dawes for technical assistance and Maria Olmedillas del Moral, Cris Richter, and Bianca Brawek for analyses of the specificity of mir-RGB vectors. We thank Professor Mark Cragg for provision of Vav-Bcl2 mice, and Dr. Salah Elias for provision of RosamT/mG mice. The research was funded by the Leverhulme Trust (RPG-2016-311), the Medical Research Council (MR/P024572/1), and the Deutsche Forschungsgemeinschaft (SFB841/SP01 to K.R. and B.F.). D.G.-N. designed the study, secured the funding, and supervised the project. L.B.-C. performed most of experiments and analyzed the data. A.R.M. and K.E.A. contributed experimental work and analysis. K.R. and B.F. provided SFFV-RGB vectors. K.L. and O.G. provided the mir-RGB vectors. P.G. performed mathematical modeling of the data. L.B.-C. D.A.M. and D.G.-N. wrote the manuscript. All authors contributed to drafting and read and approved the final manuscript. The authors declare no competing interests. We support inclusive, diverse, and equitable conduct of research. Funding Information: We thank the Southampton Flow Cytometry Facility and the Imaging Unit for technical advice and the Biomedical Research Facility for assistance with animal breeding and maintenance. We thank Georgina Dawes for technical assistance and Maria Olmedillas del Moral, Cris Richter, and Bianca Brawek for analyses of the specificity of mir-RGB vectors. We thank Professor Mark Cragg for provision of Vav-Bcl2 mice, and Dr. Salah Elias for provision of Rosa mT/mG mice. The research was funded by the Leverhulme Trust ( RPG-2016-311 ), the Medical Research Council ( MR/P024572/1 ), and the Deutsche Forschungsgemeinschaft ( SFB841/SP01 to K.R. and B.F.). Publisher Copyright: © 2023 The Author(s)

Keywords: CP: Immunology, CP: Neuroscience, RGB, brain development, clonality, fate mapping, microglia, neareast neighbour distance

Local EPrints ID: 473429

URI: http://eprints.soton.ac.uk/id/eprint/473429

DOI: doi:10.1016/j.celrep.2023.112425

ISSN: 2211-1247

PURE UUID: 01e42b5e-254a-4122-86e5-841d5970e3f9

ORCID for Philip Greulich: orcid.org/0000-0001-5247-6738

ORCID for Diego Gomez-Nicola: orcid.org/0000-0002-5316-2682

Date deposited: 18 Jan 2023 17:30

Last modified: 06 Jun 2024 01:52