A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel
A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel
Septic arthritis is a serious condition with significant morbidity and mortality, routinely diagnosed using culture. The FDA has recently approved the rapid molecular BioFire® Joint Infection Panel (BJIP) for synovial fluid. We aimed to evaluate the BJIP compared to culture and its potential use in patient management. A multicentre retrospective evaluation of BJIP was conducted in the UK and Ireland. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated between the BJIP and routine culture. A multidisciplinary team (MDT) discussion addressing the optimal or potential case use of the assay practice was facilitated. Three hundred ninety-nine surplus synovial fluid samples (~ 70% from native joints) from eight centres were processed using BJIP in addition to routine culture. An increased yield of positive results was detected using BJIP compared to routine culture (98 vs 83), giving an overall PPA of 91.6% and overall NPA of 93% for the BJIP compared to culture results. The BJIP detected resistant markers and additional organisms that could influence antibiotic choices including Neisseria gonorrhoeae and Kingella kingae. The MDT agreed that the assay could be used, in addition to standard methods, in adult and children patients with specialist advice use based on local needs. Rapid results from BJIP were assessed as having potential clinical impact on patient management. Organisms not included in the panel may be clinically significant and may limit the value of this test for PJI.
PCR, Prosthetic joint infections, Rapid diagnosis, Septic arthritis
169–176
Saeed, Kordo
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Ahmad-Saeed, Nusreen
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Annett, Rachel
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Barlow, Gavin
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Barrett, Lucinda
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Boyd, Sara E.
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Boran, Nicola
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Davies, Peter
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Hughes, Harriet
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Jones, Gwennan
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Leach, Laura
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Lynch, Maureen
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Nayar, Deepa
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Maloney, Robert J.
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Marsh, Martin
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Milburn, Olivia
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Mitchell, Shanine
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Moffat, Lynn
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Moore, Luke S. P.
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Murphy, Michael E.
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O’Shea, Shaan Ashk
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O’Sullivan, Fionnuala
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Peach, Teresa
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Petridou, Christina
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Reidy, Niamh
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Selvaratnam, Mathyruban
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Talbot, Ben
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Taylor, Vanessa
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Wearmouth, Deborah
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Aldridge, Catherine
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Saeed, Kordo
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Ahmad-Saeed, Nusreen
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Annett, Rachel
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Barlow, Gavin
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Barrett, Lucinda
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Boran, Nicola
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Jones, Gwennan
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Leach, Laura
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Lynch, Maureen
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Nayar, Deepa
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Maloney, Robert J.
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Marsh, Martin
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Murphy, Michael E.
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O’Shea, Shaan Ashk
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O’Sullivan, Fionnuala
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Peach, Teresa
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Petridou, Christina
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Selvaratnam, Mathyruban
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Talbot, Ben
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Taylor, Vanessa
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Wearmouth, Deborah
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Aldridge, Catherine
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Saeed, Kordo, Ahmad-Saeed, Nusreen, Annett, Rachel, Barlow, Gavin, Barrett, Lucinda, Boyd, Sara E., Boran, Nicola, Davies, Peter, Hughes, Harriet, Jones, Gwennan, Leach, Laura, Lynch, Maureen, Nayar, Deepa, Maloney, Robert J., Marsh, Martin, Milburn, Olivia, Mitchell, Shanine, Moffat, Lynn, Moore, Luke S. P., Murphy, Michael E., O’Shea, Shaan Ashk, O’Sullivan, Fionnuala, Peach, Teresa, Petridou, Christina, Reidy, Niamh, Selvaratnam, Mathyruban, Talbot, Ben, Taylor, Vanessa, Wearmouth, Deborah and Aldridge, Catherine
(2022)
A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel.
European Journal of Clinical Microbiology & Infectious Diseases, 42, .
(doi:10.1007/s10096-022-04538-w).
Abstract
Septic arthritis is a serious condition with significant morbidity and mortality, routinely diagnosed using culture. The FDA has recently approved the rapid molecular BioFire® Joint Infection Panel (BJIP) for synovial fluid. We aimed to evaluate the BJIP compared to culture and its potential use in patient management. A multicentre retrospective evaluation of BJIP was conducted in the UK and Ireland. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated between the BJIP and routine culture. A multidisciplinary team (MDT) discussion addressing the optimal or potential case use of the assay practice was facilitated. Three hundred ninety-nine surplus synovial fluid samples (~ 70% from native joints) from eight centres were processed using BJIP in addition to routine culture. An increased yield of positive results was detected using BJIP compared to routine culture (98 vs 83), giving an overall PPA of 91.6% and overall NPA of 93% for the BJIP compared to culture results. The BJIP detected resistant markers and additional organisms that could influence antibiotic choices including Neisseria gonorrhoeae and Kingella kingae. The MDT agreed that the assay could be used, in addition to standard methods, in adult and children patients with specialist advice use based on local needs. Rapid results from BJIP were assessed as having potential clinical impact on patient management. Organisms not included in the panel may be clinically significant and may limit the value of this test for PJI.
Text
s10096-022-04538-w
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e-pub ahead of print date: 7 December 2022
Additional Information:
Funding Information:
Mairi Macleod and Sandra Higgins at the Department of Microbiology, NHS Greater Glasgow and Clyde, Glasgow Royal Infirmary, UK LSPM acknowledge support from the NIHR, Imperial Biomedical Research Centre, and the NIHR Health Protection Research Unit in health-care-associated infection and antimicrobial resistance at Imperial College London, in partnership with Public Health England. The views expressed in this correspondence are those of the authors, and not necessarily those of the National Health Service, the NIHR, or the UK Department of Health. KS would like to thank laboratory development group (LD) and laboratory management group (LMG) at the University Hospital Southampton for their support throughout the evaluation.
Funding Information:
KS has received research grants and speaker fees from Pfizer, Thermo Fisher, and Menarini. LSPM has consulted for and received speaker fees from bioMerieux, Pfizer, Eumedica, Shionogi, Pulmocide, Umovis Labs, DNA Electronics, Kent Pharma, and Sumitovant and received research grants from the UK National Institute for Health Research (NIHR), LifeArc, and the CW + charity. SEB held research funding through an MRC RCUK/UKRI Innovation Fellowship (MR/R016895/1) and the North West MRC Scheme in Clinical Pharmacology (MR/N025989/1). She received research support from Roche Pharma and has consulted for/received speaker fees from Sumitovant and Shionogi. CA received speaker fees from bioMerieux.
Publisher Copyright:
© 2022, The Author(s).
Keywords:
PCR, Prosthetic joint infections, Rapid diagnosis, Septic arthritis
Identifiers
Local EPrints ID: 473547
URI: http://eprints.soton.ac.uk/id/eprint/473547
ISSN: 0934-9723
PURE UUID: 4517235a-0927-47f0-ad1a-9dac1db628e5
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Date deposited: 23 Jan 2023 17:44
Last modified: 17 Mar 2024 03:56
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Contributors
Author:
Kordo Saeed
Author:
Nusreen Ahmad-Saeed
Author:
Rachel Annett
Author:
Gavin Barlow
Author:
Lucinda Barrett
Author:
Sara E. Boyd
Author:
Nicola Boran
Author:
Peter Davies
Author:
Harriet Hughes
Author:
Gwennan Jones
Author:
Laura Leach
Author:
Maureen Lynch
Author:
Deepa Nayar
Author:
Robert J. Maloney
Author:
Martin Marsh
Author:
Olivia Milburn
Author:
Shanine Mitchell
Author:
Lynn Moffat
Author:
Luke S. P. Moore
Author:
Michael E. Murphy
Author:
Shaan Ashk O’Shea
Author:
Fionnuala O’Sullivan
Author:
Teresa Peach
Author:
Christina Petridou
Author:
Niamh Reidy
Author:
Mathyruban Selvaratnam
Author:
Ben Talbot
Author:
Vanessa Taylor
Author:
Deborah Wearmouth
Author:
Catherine Aldridge
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