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Model and Image based Investigation into Mechanisms of Olive Dieback caused by Xylella fastidiosa biofilms

Model and Image based Investigation into Mechanisms of Olive Dieback caused by Xylella fastidiosa biofilms
Model and Image based Investigation into Mechanisms of Olive Dieback caused by Xylella fastidiosa biofilms
Xylella fastidiosa (X. fastidiosa) is a bacterium that colonises internal plant vascular networks causing pathogenic effects on several commercially important crops (e.g. olives, grapes, coffee, etc.). Despite a growing research effort since the recent detection of X. fastidiosa in Europe, the exact processes leading to X. fastidiosa disease symptoms are not fully understood due to difficulties in observing internal plant structures. Our goal is to utilise models to elucidate fundamental processes that lead to olive quick decline syndrome, the disease responsible for the devastating olive decline in Apulia, Southern Italy. We endeavour to do this by developing a fundamental mathematical modelling framework describing within-host biofilm development, and the resulting water-stresses that ultimately inhibit plant functionality and development. We have begun work on developing a fluid mechanics model that invokes a polymer-physical description of X.fastidiosa biofilm formation dynamics. Our approach is centred on the assumption that the biofilm structure is determined by the arrangement of extracellular polysaccharide (EPS) molecules. We have also produced X-ray Computed Tomography (XCT) images of both resistant and susceptible olive cultivars to determine possible morphological differences between the vascular networks of the cultivars. Additionally, these images provide detailed geometries for our models, with which we will investigate the effect of xylem geometry on resistance. Preliminary modelling results of EPS dominant biofilm formation characterise biofilm coalescence in a consistent manner with early microfluidic controlled experiments and require fewer empirical assumptions. We hope our work will improve the understanding of possible cultivar resistance mechanisms to enable informed breeding and effective replanting in Apulia, and that model simulations will provide insights for improving the efficiency of current sampling techniques.<br/
Walker, Nancy Catherine
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White, S M
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Mckay Fletcher, Daniel
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Ruiz, Siul Aljadi
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Rankin, Kathryn E.
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De Stradis, A
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Saponari, M
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Williams, Katherine Anne
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Petroselli, Chiara
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Roose, Tiina
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Walker, Nancy Catherine
0b539663-b1db-4e93-a513-2580c3229df4
White, S M
6345bc58-972b-4a3b-a520-46c541ef5d37
Mckay Fletcher, Daniel
db06e7e0-69af-4fa2-89b3-26f6599e43d4
Ruiz, Siul Aljadi
d79b3b82-7c0d-47cc-9616-11d29e6a41bd
Rankin, Kathryn E.
d9516566-0ad8-473d-b99b-4683c663a2b7
De Stradis, A
44fb8ccc-9e5a-4abc-ba29-82b6139a7853
Saponari, M
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Williams, Katherine Anne
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Petroselli, Chiara
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Roose, Tiina
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Walker, Nancy Catherine, White, S M, Mckay Fletcher, Daniel, Ruiz, Siul Aljadi, Rankin, Kathryn E., De Stradis, A, Saponari, M, Williams, Katherine Anne, Petroselli, Chiara and Roose, Tiina (2022) Model and Image based Investigation into Mechanisms of Olive Dieback caused by Xylella fastidiosa biofilms. 3rd European Conference on Xylella fastidosa and XF-ACTORS final meeting (xylella21). 26 - 30 Apr 2021. 1 pp .

Record type: Conference or Workshop Item (Paper)

Abstract

Xylella fastidiosa (X. fastidiosa) is a bacterium that colonises internal plant vascular networks causing pathogenic effects on several commercially important crops (e.g. olives, grapes, coffee, etc.). Despite a growing research effort since the recent detection of X. fastidiosa in Europe, the exact processes leading to X. fastidiosa disease symptoms are not fully understood due to difficulties in observing internal plant structures. Our goal is to utilise models to elucidate fundamental processes that lead to olive quick decline syndrome, the disease responsible for the devastating olive decline in Apulia, Southern Italy. We endeavour to do this by developing a fundamental mathematical modelling framework describing within-host biofilm development, and the resulting water-stresses that ultimately inhibit plant functionality and development. We have begun work on developing a fluid mechanics model that invokes a polymer-physical description of X.fastidiosa biofilm formation dynamics. Our approach is centred on the assumption that the biofilm structure is determined by the arrangement of extracellular polysaccharide (EPS) molecules. We have also produced X-ray Computed Tomography (XCT) images of both resistant and susceptible olive cultivars to determine possible morphological differences between the vascular networks of the cultivars. Additionally, these images provide detailed geometries for our models, with which we will investigate the effect of xylem geometry on resistance. Preliminary modelling results of EPS dominant biofilm formation characterise biofilm coalescence in a consistent manner with early microfluidic controlled experiments and require fewer empirical assumptions. We hope our work will improve the understanding of possible cultivar resistance mechanisms to enable informed breeding and effective replanting in Apulia, and that model simulations will provide insights for improving the efficiency of current sampling techniques.<br/

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Published date: 12 December 2022
Venue - Dates: 3rd European Conference on Xylella fastidosa and XF-ACTORS final meeting (xylella21), 2021-04-26 - 2021-04-30

Identifiers

Local EPrints ID: 473558
URI: http://eprints.soton.ac.uk/id/eprint/473558
PURE UUID: f5a55637-5b62-4e83-b4cc-d8419e54ce08
ORCID for Nancy Catherine Walker: ORCID iD orcid.org/0000-0003-2297-1046
ORCID for Daniel Mckay Fletcher: ORCID iD orcid.org/0000-0001-6569-2931
ORCID for Kathryn E. Rankin: ORCID iD orcid.org/0000-0002-8458-1038
ORCID for Katherine Anne Williams: ORCID iD orcid.org/0000-0001-6827-9261
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 23 Jan 2023 17:51
Last modified: 04 Jun 2024 01:58

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Contributors

Author: S M White
Author: Daniel Mckay Fletcher ORCID iD
Author: Kathryn E. Rankin ORCID iD
Author: A De Stradis
Author: M Saponari
Author: Katherine Anne Williams ORCID iD
Author: Chiara Petroselli
Author: Tiina Roose ORCID iD

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