Nanocomposite clay-based bioinks for skeletal tissue engineering
Nanocomposite clay-based bioinks for skeletal tissue engineering
Biofabrication is revolutionizing substitute tissue manufacturing. Skeletal stem cells (SSCs) can be blended with hydrogel biomaterials and printed to form three-dimensional structures that can closely mimic tissues of interest. Our bioink formulation takes into account the potential for cell printing including a bioink nanocomposite that contains low fraction polymeric content to facilitate cell encapsulation and survival, while preserving hydrogel integrity and mechanical properties following extrusion. Clay inclusion to the nanocomposite strengthens the alginate-methylcellulose network providing a biopaste with unique shear-thinning properties that can be easily prepared under sterile conditions. SSCs can be mixed with the clay-based paste, and the resulting bioink can be printed in 3D structures ready for implantation. In this chapter, we provide the methodology for preparation, encapsulation, and printing of SSCs in a unique clay-based bioink.
63-72
Cidonio, Gianluca
558ad583-899a-4d8c-b42b-bc1c354c8757
Glinka, Michael
7630ab6c-91c5-4840-9c25-12cb61fcb91e
Kim, Yang-Hee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
2021
Cidonio, Gianluca
558ad583-899a-4d8c-b42b-bc1c354c8757
Glinka, Michael
7630ab6c-91c5-4840-9c25-12cb61fcb91e
Kim, Yang-Hee
de0d641b-c2cb-4e73-9ae2-e20d33689f5d
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, Richard O. C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Cidonio, Gianluca, Glinka, Michael, Kim, Yang-Hee, Dawson, Jonathan I. and Oreffo, Richard O. C.
(2021)
Nanocomposite clay-based bioinks for skeletal tissue engineering.
In,
Methods in Molecular Biology.
.
(doi:10.1007/978-1-0716-0611-7_6).
Record type:
Book Section
Abstract
Biofabrication is revolutionizing substitute tissue manufacturing. Skeletal stem cells (SSCs) can be blended with hydrogel biomaterials and printed to form three-dimensional structures that can closely mimic tissues of interest. Our bioink formulation takes into account the potential for cell printing including a bioink nanocomposite that contains low fraction polymeric content to facilitate cell encapsulation and survival, while preserving hydrogel integrity and mechanical properties following extrusion. Clay inclusion to the nanocomposite strengthens the alginate-methylcellulose network providing a biopaste with unique shear-thinning properties that can be easily prepared under sterile conditions. SSCs can be mixed with the clay-based paste, and the resulting bioink can be printed in 3D structures ready for implantation. In this chapter, we provide the methodology for preparation, encapsulation, and printing of SSCs in a unique clay-based bioink.
This record has no associated files available for download.
More information
e-pub ahead of print date: 26 August 2020
Published date: 2021
Identifiers
Local EPrints ID: 473653
URI: http://eprints.soton.ac.uk/id/eprint/473653
PURE UUID: 8325088f-addf-4953-b62d-820aab8bf738
Catalogue record
Date deposited: 26 Jan 2023 17:46
Last modified: 17 Mar 2024 03:41
Export record
Altmetrics
Contributors
Author:
Gianluca Cidonio
Author:
Michael Glinka
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
