Systematic review and meta-analysis: water type and temperature affect environmental DNA decay
Systematic review and meta-analysis: water type and temperature affect environmental DNA decay
Environmental DNA (eDNA) has been used in a variety of ecological studies and management applications. The rate at which eDNA decays has been widely studied but at present it is difficult to disentangle study-specific effects from factors that universally affect eDNA degradation. To address this, a systematic review and meta-analysis was conducted on aquatic eDNA studies. Analysis revealed eDNA decayed faster at higher temperatures and in marine environments (as opposed to freshwater). DNA type (mitochondrial or nuclear) and fragment length did not affect eDNA decay rate, although a preference for <200 bp sequences in the available literature means this relationship was not assessed with longer sequences (e.g. >800 bp). At present, factors such as ultraviolet light, pH, and microbial load lacked sufficient studies to feature in the meta-analysis. Moving forward, we advocate researching these factors to further refine our understanding of eDNA decay in aquatic environments.
2494-2505
Lamb, Philip D.
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Fonseca, Vera G.
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Maxwell, David L.
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Nnanatu, Chibuzor Christopher
24be7c1b-a677-4086-91b4-a9d9b1efa5a3
5 May 2022
Lamb, Philip D.
840ac742-331b-45b4-a9a9-72b3daf5d940
Fonseca, Vera G.
6fabd0f1-4108-425e-ae17-d80cbaefcce8
Maxwell, David L.
c9e26213-b0b2-4a64-ae0b-31031db7953e
Nnanatu, Chibuzor Christopher
24be7c1b-a677-4086-91b4-a9d9b1efa5a3
Lamb, Philip D., Fonseca, Vera G., Maxwell, David L. and Nnanatu, Chibuzor Christopher
(2022)
Systematic review and meta-analysis: water type and temperature affect environmental DNA decay.
Molecular Ecology Resources, 22 (7), , [7].
(doi:10.1111/1755-0998.13627).
Abstract
Environmental DNA (eDNA) has been used in a variety of ecological studies and management applications. The rate at which eDNA decays has been widely studied but at present it is difficult to disentangle study-specific effects from factors that universally affect eDNA degradation. To address this, a systematic review and meta-analysis was conducted on aquatic eDNA studies. Analysis revealed eDNA decayed faster at higher temperatures and in marine environments (as opposed to freshwater). DNA type (mitochondrial or nuclear) and fragment length did not affect eDNA decay rate, although a preference for <200 bp sequences in the available literature means this relationship was not assessed with longer sequences (e.g. >800 bp). At present, factors such as ultraviolet light, pH, and microbial load lacked sufficient studies to feature in the meta-analysis. Moving forward, we advocate researching these factors to further refine our understanding of eDNA decay in aquatic environments.
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Molecular Ecology Resources - 2022 - Lamb - Systematic review and meta‐analysis Water type and temperature affect
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Accepted/In Press date: 17 April 2022
Published date: 5 May 2022
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Local EPrints ID: 473799
URI: http://eprints.soton.ac.uk/id/eprint/473799
ISSN: 1755-098X
PURE UUID: 12421391-0eca-4013-a8e0-6cd71a434edb
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Date deposited: 31 Jan 2023 17:52
Last modified: 16 Mar 2024 23:52
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Contributors
Author:
Philip D. Lamb
Author:
Vera G. Fonseca
Author:
David L. Maxwell
Author:
Chibuzor Christopher Nnanatu
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