Conjugated linoleic acids have anti-inflammatory effects in cultured endothelial cells
Conjugated linoleic acids have anti-inflammatory effects in cultured endothelial cells
Conjugated linoleic acid (CLA) isomers may have a role in preventing atherosclerosis through the modulation of inflammation, particularly of the endothelium. However, whether low concentrations of CLAs are able to affect basal unstimulated endothelial cell (EC) responses is not clear. The aim of this study was to evaluate the effects of two CLAs (cis-9, trans-11 (CLA9,11) and trans-10, cis-12 (CLA10,12)) on the basal inflammatory responses by ECs. EA.hy926 cells (HUVEC lineage) were cultured under standard conditions and exposed to individual CLAs for 48 h. Both CLAs were incorporated into ECs in a dose-dependent manner. CLA9,11 (1 μM) significantly decreased concentrations of MCP-1 (p < 0.05), IL-6 (p < 0.05), IL-8 (p < 0.01) and RANTES (p < 0.05) in the culture medium. CLA10,12 (10 μM) decreased the concentrations of MCP-1 (p < 0.05) and RANTES (p < 0.05) but increased the concentration of IL-6 (p < 0.001). At 10 μM both CLAs increased the relative expression of the NFκβ subunit 1 gene (p < 0.01 and p < 0.05, respectively), while decreasing the relative expression of PPARα (p < 0.0001), COX-2 (p < 0.0001) and IL-6 (p < 0.0001) genes. CLA10,12 increased the relative expression of the gene encoding IκK-β at 10 μM compared with CLA9,11 (p < 0.05) and increased the relative expression of the gene encoding IκBα at 1 and 10 μM compared with linoleic acid (both p < 0.05). Neither CLA affected the adhesion of monocytes to ECs. These results suggest that low concentrations of both CLA9,11 and CLA10,12 have modest anti-inflammatory effects in ECs. Thus, CLAs may influence endothelial function and the risk of vascular disease. Nevertheless, at these low CLA concentrations some pro-inflammatory genes are upregulated while others are downregulated, suggesting complex effects of CLAs on inflammatory pathways.
atherosclerosis, conjugated fatty acids, endothelial cells, inflammation
Valenzuela, Carina A.
1a12a9b9-6504-4392-90c5-246644b0ad5c
Baker, Ella J.
7cd5b762-d7d7-4584-b9a7-dba555085440
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
3 January 2023
Valenzuela, Carina A.
1a12a9b9-6504-4392-90c5-246644b0ad5c
Baker, Ella J.
7cd5b762-d7d7-4584-b9a7-dba555085440
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Valenzuela, Carina A., Baker, Ella J., Miles, Elizabeth A. and Calder, Philip C.
(2023)
Conjugated linoleic acids have anti-inflammatory effects in cultured endothelial cells.
International Journal of Molecular Sciences, 24 (1), [874].
(doi:10.3390/ijms24010874).
Abstract
Conjugated linoleic acid (CLA) isomers may have a role in preventing atherosclerosis through the modulation of inflammation, particularly of the endothelium. However, whether low concentrations of CLAs are able to affect basal unstimulated endothelial cell (EC) responses is not clear. The aim of this study was to evaluate the effects of two CLAs (cis-9, trans-11 (CLA9,11) and trans-10, cis-12 (CLA10,12)) on the basal inflammatory responses by ECs. EA.hy926 cells (HUVEC lineage) were cultured under standard conditions and exposed to individual CLAs for 48 h. Both CLAs were incorporated into ECs in a dose-dependent manner. CLA9,11 (1 μM) significantly decreased concentrations of MCP-1 (p < 0.05), IL-6 (p < 0.05), IL-8 (p < 0.01) and RANTES (p < 0.05) in the culture medium. CLA10,12 (10 μM) decreased the concentrations of MCP-1 (p < 0.05) and RANTES (p < 0.05) but increased the concentration of IL-6 (p < 0.001). At 10 μM both CLAs increased the relative expression of the NFκβ subunit 1 gene (p < 0.01 and p < 0.05, respectively), while decreasing the relative expression of PPARα (p < 0.0001), COX-2 (p < 0.0001) and IL-6 (p < 0.0001) genes. CLA10,12 increased the relative expression of the gene encoding IκK-β at 10 μM compared with CLA9,11 (p < 0.05) and increased the relative expression of the gene encoding IκBα at 1 and 10 μM compared with linoleic acid (both p < 0.05). Neither CLA affected the adhesion of monocytes to ECs. These results suggest that low concentrations of both CLA9,11 and CLA10,12 have modest anti-inflammatory effects in ECs. Thus, CLAs may influence endothelial function and the risk of vascular disease. Nevertheless, at these low CLA concentrations some pro-inflammatory genes are upregulated while others are downregulated, suggesting complex effects of CLAs on inflammatory pathways.
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Accepted/In Press date: 31 December 2022
Published date: 3 January 2023
Additional Information:
Funding Information:
C.A.V. was supported by CONICYT (Comisión Nacional de Investigación Científica y Tecnológica, Gobierno de Chile) through its scholarship program Becas Chile. E.J.B. was supported by the Biotechnology and Biological Sciences Research Council under the Food Security Doctoral Training Programme and by the Faculty of Medicine, University of Southampton.
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© 2023 by the authors.
Keywords:
atherosclerosis, conjugated fatty acids, endothelial cells, inflammation
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Local EPrints ID: 473837
URI: http://eprints.soton.ac.uk/id/eprint/473837
ISSN: 1422-0067
PURE UUID: c17cfaa0-9e8b-4bce-b7c4-d9e837dd7972
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Date deposited: 01 Feb 2023 17:40
Last modified: 17 Mar 2024 03:55
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Author:
Carina A. Valenzuela
Author:
Ella J. Baker
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