Sensitive absorbance measurement in droplet microfluidics via multipass flow cells
Sensitive absorbance measurement in droplet microfluidics via multipass flow cells
Absorbance measurement is a useful analytical tool that can be used along with colorimetric assays to measure a wide range of analytes. However, since sensitivity is directly proportional to path length, sensitive measurements in microfluidic channels are inherently challenging. This is especially true for droplet microfluidics where the lensing at the droplet/carrier interface further constrains path length. In this study, we developed multipass flow cells assembled with squared PTFE tube with parallel mirrors on both sides, laser diode and detector. The devices featured affordable low power components, and was made using simple fabrication techniques making it accessible to a wide range of researchers. In testing it allowed multiple reflection of light in the detection chamber, which significantly increased the optical path length by 8 times. The flow cell was used to quantify the phosphate levels in water samples from a tidal chalk river which could not be measured with a simple single-pass flow cell.
Lu, Bingyuan
124a3e2b-15b5-4013-8f14-39a65f01c2a7
Nightingale, Adrian
4b51311d-c6c3-40d5-a13f-ab8917031ab3
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
10 October 2021
Lu, Bingyuan
124a3e2b-15b5-4013-8f14-39a65f01c2a7
Nightingale, Adrian
4b51311d-c6c3-40d5-a13f-ab8917031ab3
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
Lu, Bingyuan, Nightingale, Adrian and Niu, Xize
(2021)
Sensitive absorbance measurement in droplet microfluidics via multipass flow cells.
The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021), , Palm Springs, CA, United States.
10 - 14 Oct 2021.
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Conference or Workshop Item
(Other)
Abstract
Absorbance measurement is a useful analytical tool that can be used along with colorimetric assays to measure a wide range of analytes. However, since sensitivity is directly proportional to path length, sensitive measurements in microfluidic channels are inherently challenging. This is especially true for droplet microfluidics where the lensing at the droplet/carrier interface further constrains path length. In this study, we developed multipass flow cells assembled with squared PTFE tube with parallel mirrors on both sides, laser diode and detector. The devices featured affordable low power components, and was made using simple fabrication techniques making it accessible to a wide range of researchers. In testing it allowed multiple reflection of light in the detection chamber, which significantly increased the optical path length by 8 times. The flow cell was used to quantify the phosphate levels in water samples from a tidal chalk river which could not be measured with a simple single-pass flow cell.
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Published date: 10 October 2021
Venue - Dates:
The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021), , Palm Springs, CA, United States, 2021-10-10 - 2021-10-14
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Local EPrints ID: 474150
URI: http://eprints.soton.ac.uk/id/eprint/474150
PURE UUID: 4a827131-913a-4310-a485-e732f1e8389b
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Date deposited: 14 Feb 2023 17:41
Last modified: 18 Nov 2023 02:45
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Author:
Bingyuan Lu
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