READ ME File For 'Single-cell RNA sequencing datasets from human foetal cardiac fibroblasts'

Dataset DOI: 10.5258/SOTON/D2533

ReadMe Author: MARIA GABRIELA DE OLIVEIRA BARBETA, University of Southampton ORCID ID: https://orcid.org/0000-0001-6848-8581

This dataset supports the thesis entitled 'Investigating the role of NR2F2 in fibroblast during human heart development'
AWARDED BY: University of Southampton
DATE OF AWARD: 2023

DESCRIPTION OF THE DATA
The h5ad files are derived from single-cell RNA sequencing of human foetal fibroblast cells isolated from the heart, using the 10x Genomics platform for preparation and encapsulation of the cells. The sequencing was done in the Illumina platform. 
The h5ad files contain the cell x genes matrix, pre-filtered and tagged using Scanpy tools. 

This dataset contains:
1.FB_clean_taged_2.h5ad: H5ad file of pre-filtered, tagged cells derived from single-cell RNA sequencing of primary foetal fibroblasts isolated from human hearts. This data was analysed using the codes inserted in the Appendix A and the data was exposed in Chapter 3 of the PhD thesis entitled: “Investigating the role of NR2F2 in fibroblast during human heart development”

2.CRISPR_clean_taged.h5ad: H5ad file of pre-filtered, tagged cells derived from single-cell RNA sequencing of primary foetal fibroblasts isolated from human hearts and MRC-5 cell line, after submitted to gene editing in NR2F2 gene loci using CRISPR/Cas9 method. This data was analysed using the codes inserted in the Appendix C and the data was exposed in Chapter 6 of the PhD thesis entitled: “Investigating the role of NR2F2 in fibroblast during human heart development”

Date of data collection: 2021

Licence:
CC BY

Related projects/Funders:
CNPq (Brazil) - 207483/2017-5

Related publication:
Phd thesis:'Investigating the role of NR2F2 in fibroblast during human heart development'


Date that the file was created: February, 2023