The outcome of neutrophil-T cell contact differs depending on activation status of both cell types

Minns, Danielle, Smith, Katie J., Hardisty, Gareth, Rossi, Adriano G. and Gwyer, Findlay E (2021) The outcome of neutrophil-T cell contact differs depending on activation status of both cell types. Frontiers in Immunology, 2021 (3), [633486]. (doi:10.3389/fimmu.2021.633486).

Abstract

Neutrophils and T cells exist in close proximity in lymph nodes and inflamed tissues duringhealth and disease. They are able to form stable interactions, with profound effects on thephenotype and function of the T cells. However, the outcome of these effects arefrequently contradictory; in some systems neutrophils suppress T cell proliferation, inothers they are activatory or present antigen directly. Published protocols modelling theseinteractions in vitro do not reflect the full range of interactions found in vivo; they do notexamine how activated and naïve T cells differentially respond to neutrophils, or whetherde-granulating or resting neutrophils induce different outcomes. Here, we established aculture protocol to ask these questions with human T cells and autologous neutrophils.We find that resting neutrophils suppress T cell proliferation, activation and cytokineproduction but that de-granulating neutrophils do not, and neutrophil-releasedintracellular contents enhance proliferation. Strikingly, we also demonstrate that T cellsearly in the activation process are susceptible to suppression by neutrophils, while laterstage T cells are not, and naïve T cells do not respond at all. Our protocol therefore allowsnuanced analysis of the outcome of interaction of these cells and may explain thecontradictory results observed previously.

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Contributors

Author: Findlay E Gwyer

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