Anti-Group B Streptococcus antibody in infants born to mothers with human immunodeficiency virus (HIV) infection
Anti-Group B Streptococcus antibody in infants born to mothers with human immunodeficiency virus (HIV) infection
Background: HIV-exposed uninfected infants have increased infection risk and mortality compared to HIV-unexposed infants. HIV-exposed infants may be at increased risk of invasive GBS disease due to reduced maternal antibody against GBS.
Methods: We quantified antibodies that bind to the surface of whole Group B Streptococcus (GBS) of serotypes Ia, Ib, II, III and V using novel flow cytometry assays in South African HIV-infected and non-infected mothers and their uninfected infants. Antibody-mediated complement C3b/iC3b deposition onto GBS of these serotypes was also quantified by a novel flow cytometry assay. Results: Geometric mean concentration (GMC) of both surface-binding anti-GBS antibody and antibody-mediated complement deposition onto GBS were reduced in HIV-infected women (n= 46) compared to HIV-uninfected women (n= 58) for ST1a (surface-binding: 19.3 vs 29.3; p= 0.003; complement deposition: 2.9 vs 5.3. SU/mL; p= 0.003), STIb (24.9 vs 47.6; p= 0.003; 2.6 vs 4.9. SU/mL; p= 0.003), STII (19.8 vs 50.0; p= 0.001; 3.1 vs 6.2. SU/mL; p= 0.001), STIII (27.8 vs 60.1; p= 0.001; 2.8 vs 5.3. SU/mL; p= 0.001) and STV (121.9 vs 185.6. SU/mL; p<. 0.001) and in their infants for STIa (complement deposition 9.4 vs 27.0. SU/mL; p= 0.02), STIb (13.4 vs 24.5. SU/mL; p= 0.02), STII (14.6 vs 42.7. SU/mL; p= 0.03), STIII (26.6 vs 62.7. SU/mL; p= 0.03) and STV (90.4 vs 165.8. SU/mL; p= 0.04). Median transplacental transfer of antibody from HIV-infected women to their infants was reduced compared to HIV-uninfected women for GBS serotypes II (0.42 [IQR 0.22-0.59] vs 1.0. SU/mL [0.42-1.66]; p<. 0.001), III (0.54 [0.31-1.03] vs 0.95. SU/mL [0.42-3.05], p= 0.05) and V (0.51 [0.28-0.79] vs 0.75. SU/mL [0.26-2.9], p= 0.04). The differences between infants remained significant at 16 weeks of age.
Conclusions: Maternal HIV infection was associated with lower anti-GBS surface binding antibody concentration and antibody-mediated C3b/iC3b deposition onto GBS bacteria of serotypes Ia, Ib, II, III and V. This may render these infants more susceptible to early and late onset GBS disease.
Antibody, Group B Streptococcus, HIV, HIV-exposed-uninfected infants, Immunity
621-627
Le Doare, Kirsty
9424dc5c-4484-41d3-86dd-29cfaffb43f5
Allen, Lauren
b485b2fa-0353-44ae-801c-1603835ab5a5
Kampmann, Beate
4490f5e3-318c-4074-bf69-4a23bd5ec100
Heath, Paul Trafford
751321bd-5245-4f79-b57a-6d3b6de61b32
Taylor, Stephen
9ee68548-2096-4d91-a122-bbde65f91efb
Hesseling, Anneke C.
f97382e1-ac66-4c60-aa47-153f2ea687df
Gorringe, Andrew
503cb828-4e75-44e9-a8af-61f814e02bc6
Jones, Christine Elizabeth
48229079-8b58-4dcb-8374-d9481fe7b426
29 January 2015
Le Doare, Kirsty
9424dc5c-4484-41d3-86dd-29cfaffb43f5
Allen, Lauren
b485b2fa-0353-44ae-801c-1603835ab5a5
Kampmann, Beate
4490f5e3-318c-4074-bf69-4a23bd5ec100
Heath, Paul Trafford
751321bd-5245-4f79-b57a-6d3b6de61b32
Taylor, Stephen
9ee68548-2096-4d91-a122-bbde65f91efb
Hesseling, Anneke C.
f97382e1-ac66-4c60-aa47-153f2ea687df
Gorringe, Andrew
503cb828-4e75-44e9-a8af-61f814e02bc6
Jones, Christine Elizabeth
48229079-8b58-4dcb-8374-d9481fe7b426
Le Doare, Kirsty, Allen, Lauren, Kampmann, Beate, Heath, Paul Trafford, Taylor, Stephen and Jones, Christine Elizabeth
,
et al.
(2015)
Anti-Group B Streptococcus antibody in infants born to mothers with human immunodeficiency virus (HIV) infection.
Vaccine, 33 (5), .
(doi:10.1016/j.vaccine.2014.12.025).
Abstract
Background: HIV-exposed uninfected infants have increased infection risk and mortality compared to HIV-unexposed infants. HIV-exposed infants may be at increased risk of invasive GBS disease due to reduced maternal antibody against GBS.
Methods: We quantified antibodies that bind to the surface of whole Group B Streptococcus (GBS) of serotypes Ia, Ib, II, III and V using novel flow cytometry assays in South African HIV-infected and non-infected mothers and their uninfected infants. Antibody-mediated complement C3b/iC3b deposition onto GBS of these serotypes was also quantified by a novel flow cytometry assay. Results: Geometric mean concentration (GMC) of both surface-binding anti-GBS antibody and antibody-mediated complement deposition onto GBS were reduced in HIV-infected women (n= 46) compared to HIV-uninfected women (n= 58) for ST1a (surface-binding: 19.3 vs 29.3; p= 0.003; complement deposition: 2.9 vs 5.3. SU/mL; p= 0.003), STIb (24.9 vs 47.6; p= 0.003; 2.6 vs 4.9. SU/mL; p= 0.003), STII (19.8 vs 50.0; p= 0.001; 3.1 vs 6.2. SU/mL; p= 0.001), STIII (27.8 vs 60.1; p= 0.001; 2.8 vs 5.3. SU/mL; p= 0.001) and STV (121.9 vs 185.6. SU/mL; p<. 0.001) and in their infants for STIa (complement deposition 9.4 vs 27.0. SU/mL; p= 0.02), STIb (13.4 vs 24.5. SU/mL; p= 0.02), STII (14.6 vs 42.7. SU/mL; p= 0.03), STIII (26.6 vs 62.7. SU/mL; p= 0.03) and STV (90.4 vs 165.8. SU/mL; p= 0.04). Median transplacental transfer of antibody from HIV-infected women to their infants was reduced compared to HIV-uninfected women for GBS serotypes II (0.42 [IQR 0.22-0.59] vs 1.0. SU/mL [0.42-1.66]; p<. 0.001), III (0.54 [0.31-1.03] vs 0.95. SU/mL [0.42-3.05], p= 0.05) and V (0.51 [0.28-0.79] vs 0.75. SU/mL [0.26-2.9], p= 0.04). The differences between infants remained significant at 16 weeks of age.
Conclusions: Maternal HIV infection was associated with lower anti-GBS surface binding antibody concentration and antibody-mediated C3b/iC3b deposition onto GBS bacteria of serotypes Ia, Ib, II, III and V. This may render these infants more susceptible to early and late onset GBS disease.
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Published date: 29 January 2015
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Conflict of interest statement: PTH is an investigator for research studies done on behalf of St Georges, University of London (London, UK) and funded by Novartis vaccines (Siena, Italy) and serves as a consultant to Novartis vaccines on GBS vaccines. KLD is funded by a Wellcome Trust Global Health Fellowship (London, UK), Royal College of Physicians Thomas Watt Eden Fellowship (London, UK) and BHIVA/Gilead Registrar Award (London, UK). BK is funded by an MRC Program grant (London, UK) and an NIHR Senior Research fellowship (London, UK). CJ was funded by ESPID (Geneva, Switzerland) and The Thrasher Research Fund (Atlanta, USA). Funding: This work is supported by a Wellcome Trust Global Health Fellowship (Grant Number KLD2013 ); The Thomas Watt Eden Fellowship (Royal College of Physicians Grant Number 01012013 ); and the Gilead/BHIVA Registrar Award. Contribution statement: KLD developed the manuscript and original research idea. LA participated in the research. PTH, BK, ST, CJ developed the original idea and substantially contributed to the development of the manuscript.
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© 2014.
Keywords:
Antibody, Group B Streptococcus, HIV, HIV-exposed-uninfected infants, Immunity
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Local EPrints ID: 474804
URI: http://eprints.soton.ac.uk/id/eprint/474804
ISSN: 0264-410X
PURE UUID: 3ba895c4-583b-4f51-90b6-836f4124540c
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Date deposited: 03 Mar 2023 17:31
Last modified: 06 Jun 2024 01:58
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Author:
Kirsty Le Doare
Author:
Lauren Allen
Author:
Beate Kampmann
Author:
Paul Trafford Heath
Author:
Anneke C. Hesseling
Author:
Andrew Gorringe
Corporate Author: et al.
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