Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition
Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition
Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.
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Patenall, Bethany Lee
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Williams, George
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Gwynne, Lauren
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Stephens, Liam J.
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Lampard, Emma V.
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Hathaway, Hollie J.
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Thet, Naing T.
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Young, Amber E.
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Sutton, J. Mark
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Short, Robert D.
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Bull, Steven D.
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James, Tony D.
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Sedgwick, Adam C.
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Jenkins, A. Toby A.
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28 December 2019
Patenall, Bethany Lee
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Williams, George
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Gwynne, Lauren
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Stephens, Liam J.
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Lampard, Emma V.
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Hathaway, Hollie J.
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Thet, Naing T.
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Young, Amber E.
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Sutton, J. Mark
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Short, Robert D.
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Bull, Steven D.
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James, Tony D.
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Sedgwick, Adam C.
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Jenkins, A. Toby A.
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Patenall, Bethany Lee, Williams, George, Gwynne, Lauren, Stephens, Liam J., Lampard, Emma V., Hathaway, Hollie J., Thet, Naing T., Young, Amber E., Sutton, J. Mark, Short, Robert D., Bull, Steven D., James, Tony D., Sedgwick, Adam C. and Jenkins, A. Toby A.
(2019)
Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition.
ChemComm, 2019 (55), .
(doi:10.1039/C9CC07759F).
Abstract
Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.
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c9cc07759f
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More information
Accepted/In Press date: 5 November 2019
e-pub ahead of print date: 2 December 2019
Published date: 28 December 2019
Identifiers
Local EPrints ID: 475244
URI: http://eprints.soton.ac.uk/id/eprint/475244
ISSN: 1359-7345
PURE UUID: 972beece-30b5-40fb-8736-37979df2ac0f
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Date deposited: 14 Mar 2023 17:48
Last modified: 17 Mar 2024 04:17
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Contributors
Author:
Bethany Lee Patenall
Author:
George Williams
Author:
Lauren Gwynne
Author:
Liam J. Stephens
Author:
Emma V. Lampard
Author:
Hollie J. Hathaway
Author:
Naing T. Thet
Author:
Amber E. Young
Author:
J. Mark Sutton
Author:
Robert D. Short
Author:
Steven D. Bull
Author:
Tony D. James
Author:
Adam C. Sedgwick
Author:
A. Toby A. Jenkins
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