Effects of sequence selective drugs on the gel mobility of a bent DNA fragment
Effects of sequence selective drugs on the gel mobility of a bent DNA fragment
The effects of various drugs on the structure of a bent DNA fragment have been investigated by studying DNA mobility in polyacrylamide gels. This DNA fragment has an anomalously slow rate of migration on account of its phased runs of adenines. Nogalamycin and echinomycin increase the gel mobility of kinetoplast DNA suggesting that the bending has been removed. Mithramycin, actinomycin, distamycin and ethidium have either no effect or cause a further reduction in mobility. These results are compared with other, non-bent DNA species which always show a decrease in gel mobility in the presence of DNA binding drugs.
1064-1070
Cons, Benjamin M.G.
521b0445-44c9-4f08-872c-959fcf430f88
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
28 September 1990
Cons, Benjamin M.G.
521b0445-44c9-4f08-872c-959fcf430f88
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Cons, Benjamin M.G. and Fox, Keith R.
(1990)
Effects of sequence selective drugs on the gel mobility of a bent DNA fragment.
Biochemical and Biophysical Research Communications, 171 (3), .
(doi:10.1016/0006-291X(90)90792-L).
Abstract
The effects of various drugs on the structure of a bent DNA fragment have been investigated by studying DNA mobility in polyacrylamide gels. This DNA fragment has an anomalously slow rate of migration on account of its phased runs of adenines. Nogalamycin and echinomycin increase the gel mobility of kinetoplast DNA suggesting that the bending has been removed. Mithramycin, actinomycin, distamycin and ethidium have either no effect or cause a further reduction in mobility. These results are compared with other, non-bent DNA species which always show a decrease in gel mobility in the presence of DNA binding drugs.
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Published date: 28 September 1990
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Local EPrints ID: 475573
URI: http://eprints.soton.ac.uk/id/eprint/475573
ISSN: 0006-291X
PURE UUID: 49408540-95ef-47d0-a081-1127436cc6d7
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Date deposited: 21 Mar 2023 17:51
Last modified: 17 Mar 2024 02:34
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Benjamin M.G. Cons
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