Methyl green and its analogues bind selectively to AT-rich regions of native DNA.
Methyl green and its analogues bind selectively to AT-rich regions of native DNA.
Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At low concentrations the ligands showed similar footprinting patterns which centred around AT-rich regions with a mild preference for hompolymeric A and T. At higher concentrations the dyes bound to almost all available DNA sites. Models, with and without intercalation are discussed to account for the specific binding.
263-270
Fox, K. R.
9da5debc-4e45-473e-ab8c-550d1104659f
Higson, S. L.
3dea71c9-a721-42b5-8271-e43ba9ff2d49
Scott, J. E.
3b08c21d-ab87-41c3-9c59-ece4b7b0521c
1992
Fox, K. R.
9da5debc-4e45-473e-ab8c-550d1104659f
Higson, S. L.
3dea71c9-a721-42b5-8271-e43ba9ff2d49
Scott, J. E.
3b08c21d-ab87-41c3-9c59-ece4b7b0521c
Fox, K. R., Higson, S. L. and Scott, J. E.
(1992)
Methyl green and its analogues bind selectively to AT-rich regions of native DNA.
European journal of histochemistry : EJH, 36 (3), .
Abstract
Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At low concentrations the ligands showed similar footprinting patterns which centred around AT-rich regions with a mild preference for hompolymeric A and T. At higher concentrations the dyes bound to almost all available DNA sites. Models, with and without intercalation are discussed to account for the specific binding.
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Published date: 1992
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Local EPrints ID: 475579
URI: http://eprints.soton.ac.uk/id/eprint/475579
ISSN: 1121-760X
PURE UUID: 69937808-fe0a-43b9-a552-1273694e1496
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Date deposited: 21 Mar 2023 17:51
Last modified: 20 Jan 2024 02:33
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Author:
S. L. Higson
Author:
J. E. Scott
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