Studies of the interaction of a meta-hydroxy analogue of hoechst 33258 with DNA by melting temperature, footprinting and high-resolution ¹H NMR spectroscopy

Ebrahimi, Seyed E.Sadat, Parkinson, John A., Fox, Keith R., McKie, James H., Barber, Jill and Douglas, Kenneth T. (1992) Studies of the interaction of a meta-hydroxy analogue of hoechst 33258 with DNA by melting temperature, footprinting and high-resolution ¹H NMR spectroscopy. Journal of the Chemical Society, Chemical Communications, (19), 1398-1399. (doi:10.1039/C39920001398).

Abstract

A new analogue of Hoechst 33258 1 with a small structural change, i.e., the phenolic OH group being located meta rather than para, has been shown by footprinting, DNA melting studies and high-field ¹H NMR spectroscopy to bind to the AATT sequences of DNA in a manner grossly similar to Hoechst 33258, although analysis by ¹H NMR spectroscopy indicates that the phenolic end of the ligand could be prised away from the minor groove relative to Hoechst 33258, and that the major groove structure of d(CGCGAATTCGCG) ₂ is significantly perturbed by the binding of this Hoechst 33258 analogue.

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Contributors

Author: Keith R. Fox

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