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Secondary (non-GpC) binding sites for actinomycin on DNA

Secondary (non-GpC) binding sites for actinomycin on DNA
Secondary (non-GpC) binding sites for actinomycin on DNA

Actinomycin D has long been known to bind selectively to the dinucleotide step GpC. We have investigated its ability to bind to other non-canonical sequences using a series of synthetic DNA fragments. DNase I footprinting experiments reveal that actinomycin can also bind well to GG (CC). Binding to this sequence and the canonical GC site is potentiated by flanking regions of (GT)n·(AC)n Weaker but specific binding to GT and AC is also evident and appears to be cooperative.

Actinomycin, Footprinting, Sequence selectivity
0167-4781
300-306
Waterloh, Kerstin
25acfef4-af28-43e0-b0a7-b9f237569c2d
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Waterloh, Kerstin
25acfef4-af28-43e0-b0a7-b9f237569c2d
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Waterloh, Kerstin and Fox, Keith R. (1992) Secondary (non-GpC) binding sites for actinomycin on DNA. BBA - Gene Structure and Expression, 1131 (3), 300-306. (doi:10.1016/0167-4781(92)90028-X).

Record type: Article

Abstract

Actinomycin D has long been known to bind selectively to the dinucleotide step GpC. We have investigated its ability to bind to other non-canonical sequences using a series of synthetic DNA fragments. DNase I footprinting experiments reveal that actinomycin can also bind well to GG (CC). Binding to this sequence and the canonical GC site is potentiated by flanking regions of (GT)n·(AC)n Weaker but specific binding to GT and AC is also evident and appears to be cooperative.

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More information

Published date: 15 July 1992
Keywords: Actinomycin, Footprinting, Sequence selectivity

Identifiers

Local EPrints ID: 475591
URI: http://eprints.soton.ac.uk/id/eprint/475591
DOI: doi:10.1016/0167-4781(92)90028-X
ISSN: 0167-4781
PURE UUID: cd3ea1c3-f0ed-4850-b0dd-075a47b2a971
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 22 Mar 2023 17:31
Last modified: 17 Mar 2024 02:34

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Contributors

Author: Kerstin Waterloh
Author: Keith R. Fox ORCID iD

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