Long wavelength TCF-based fluorescent probe for the detection of alkaline phosphatase in live cells
Long wavelength TCF-based fluorescent probe for the detection of alkaline phosphatase in live cells
A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
Gwynne, Lauren
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Sedgwick, Adam C.
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Gardiner, Jordan E.
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Williams, George
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Kim, Gyoungmi
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Lowe, John P.
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Maillard, Jean-Yves
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Jenkins, A. Toby A.
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Bull, Steven D.
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Sessler, Jonathan L.
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Yoon, Juyoung
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James, Tony D.
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30 April 2019
Gwynne, Lauren
98d6197d-32de-42a3-bd48-7e21e1b877f2
Sedgwick, Adam C.
7329dac0-8976-4885-bbe9-6edb9d1ab017
Gardiner, Jordan E.
3cdda2bd-8e0d-45c4-a9ed-ed73be5ae249
Williams, George
26810522-92ef-4b61-a766-582bf15be280
Kim, Gyoungmi
5a706621-1083-4300-87ba-30af827a6915
Lowe, John P.
d08a6bff-c43e-488f-a072-eaa5873c4cd7
Maillard, Jean-Yves
9d45e659-b5c3-403f-9814-0c0170f00863
Jenkins, A. Toby A.
e0e4ebe3-c2d6-4aae-81eb-66923f6f98dd
Bull, Steven D.
6f8a4a6d-a26d-4fba-bb20-08d48d8ea144
Sessler, Jonathan L.
94122c05-9bc9-446a-aecb-6471e6c83d15
Yoon, Juyoung
ab183629-6b4d-46c9-be8c-d091d48f44b6
James, Tony D.
5cfd8c35-9fa1-4583-934e-5bc6961f6fa5
Gwynne, Lauren, Sedgwick, Adam C., Gardiner, Jordan E., Williams, George and Jenkins, A. Toby A.
,
et al.
(2019)
Long wavelength TCF-based fluorescent probe for the detection of alkaline phosphatase in live cells.
Frontiers in Chemistry, 7 (4), [255].
(doi:10.3389/fchem.2019.00255).
Abstract
A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
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Accepted/In Press date: 1 April 2019
Published date: 30 April 2019
Identifiers
Local EPrints ID: 475638
URI: http://eprints.soton.ac.uk/id/eprint/475638
ISSN: 2296-2646
PURE UUID: 9b19aa22-47c9-48c4-bb1a-66d0cfc4987f
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Date deposited: 23 Mar 2023 17:32
Last modified: 17 Mar 2024 04:17
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Contributors
Author:
Lauren Gwynne
Author:
Adam C. Sedgwick
Author:
Jordan E. Gardiner
Author:
George Williams
Author:
Gyoungmi Kim
Author:
John P. Lowe
Author:
Jean-Yves Maillard
Author:
A. Toby A. Jenkins
Author:
Steven D. Bull
Author:
Jonathan L. Sessler
Author:
Juyoung Yoon
Author:
Tony D. James
Corporate Author: et al.
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