Interaction of mithramycin with isolated GC and CG sites
Interaction of mithramycin with isolated GC and CG sites
We have studied the interaction of the GC‐specific, minor groove‐binding ligand, mithramycin, with cloned DNA inserts containing isolated GC and CG sites flanked by regions of (AT)n and An · Tn using DNase I and hydroxyl radical footprinting. We find that mithramycin binds to GC better than CG and that AGCT is a better site than TGCA. Sites flanked by (AT)n appear to be bound better than those surrounded by An · Tn. Although no footprints are produced at T9GCA9 and T15CGA15, DNase I cleavage is enhanced with the GC sites suggesting that there is some interaction with the ligand. Mithramycin also alters the DNase I cleavage of (GA)n · (CT)n.
DNase I footprinting, Minor groove binding, Mithramycin, Sequence recognition
189-197
Carpenter, Mark L.
74410339-7665-4095-ac15-7dd42a6c1597
Cassidy, Sarah A.
2b61828b-b504-4fe4-8765-8f29f37bb110
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
September 1994
Carpenter, Mark L.
74410339-7665-4095-ac15-7dd42a6c1597
Cassidy, Sarah A.
2b61828b-b504-4fe4-8765-8f29f37bb110
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Carpenter, Mark L., Cassidy, Sarah A. and Fox, Keith R.
(1994)
Interaction of mithramycin with isolated GC and CG sites.
Journal of Molecular Recognition, 7 (3), .
(doi:10.1002/jmr.300070306).
Abstract
We have studied the interaction of the GC‐specific, minor groove‐binding ligand, mithramycin, with cloned DNA inserts containing isolated GC and CG sites flanked by regions of (AT)n and An · Tn using DNase I and hydroxyl radical footprinting. We find that mithramycin binds to GC better than CG and that AGCT is a better site than TGCA. Sites flanked by (AT)n appear to be bound better than those surrounded by An · Tn. Although no footprints are produced at T9GCA9 and T15CGA15, DNase I cleavage is enhanced with the GC sites suggesting that there is some interaction with the ligand. Mithramycin also alters the DNase I cleavage of (GA)n · (CT)n.
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Published date: September 1994
Keywords:
DNase I footprinting, Minor groove binding, Mithramycin, Sequence recognition
Identifiers
Local EPrints ID: 475667
URI: http://eprints.soton.ac.uk/id/eprint/475667
ISSN: 0952-3499
PURE UUID: 37b80d37-fce9-4199-8935-77183259278e
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Date deposited: 23 Mar 2023 17:49
Last modified: 17 Mar 2024 02:34
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Author:
Mark L. Carpenter
Author:
Sarah A. Cassidy
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