A molecular anchor for stabilizing triple-helical DNA
A molecular anchor for stabilizing triple-helical DNA
Molecular modeling has been used to predict that 2,6-disubstituted amidoanthraquinones, and not the 1,4 series, should preferentially interact with and stabilize triple-stranded DNA structures over duplex DNA. This is due to marked differences in the nature of chromophore-base stacking and groove accessibility for the two series. A DNA footprinting method that monitors the extent of protection from DNase I cleavage on triplex formation has been used to examine the effects of a number of synthetic isomer compounds in the 1,4 and 2,6 series. The experimental results are in accord with the predicted behavior and confirm that the 1,4 series bind preferentially to double- rather than triple-stranded DNA, whereas the isomeric 2,6 derivatives markedly favor binding to triplex DNA.
7887-7891
Fox, Keith R.
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Polucci, Paolo
0d9ff26a-6135-4ab6-83d7-7cceb50c8838
Jenkins, Terence C.
87f02eac-6560-4f66-92fa-c87a7d55d558
Neidle, Stephen
edf2d7ee-a257-4d4c-a3ea-b3c9c42b5ff3
15 August 1995
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Polucci, Paolo
0d9ff26a-6135-4ab6-83d7-7cceb50c8838
Jenkins, Terence C.
87f02eac-6560-4f66-92fa-c87a7d55d558
Neidle, Stephen
edf2d7ee-a257-4d4c-a3ea-b3c9c42b5ff3
Fox, Keith R., Polucci, Paolo, Jenkins, Terence C. and Neidle, Stephen
(1995)
A molecular anchor for stabilizing triple-helical DNA.
Proceedings of the National Academy of Sciences of the United States of America, 92 (17), .
(doi:10.1073/pnas.92.17.7887).
Abstract
Molecular modeling has been used to predict that 2,6-disubstituted amidoanthraquinones, and not the 1,4 series, should preferentially interact with and stabilize triple-stranded DNA structures over duplex DNA. This is due to marked differences in the nature of chromophore-base stacking and groove accessibility for the two series. A DNA footprinting method that monitors the extent of protection from DNase I cleavage on triplex formation has been used to examine the effects of a number of synthetic isomer compounds in the 1,4 and 2,6 series. The experimental results are in accord with the predicted behavior and confirm that the 1,4 series bind preferentially to double- rather than triple-stranded DNA, whereas the isomeric 2,6 derivatives markedly favor binding to triplex DNA.
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Published date: 15 August 1995
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Local EPrints ID: 475672
URI: http://eprints.soton.ac.uk/id/eprint/475672
ISSN: 0027-8424
PURE UUID: c58fecbe-22bc-4a50-9243-9365bf89a541
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Date deposited: 23 Mar 2023 17:50
Last modified: 17 Mar 2024 02:34
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Author:
Paolo Polucci
Author:
Terence C. Jenkins
Author:
Stephen Neidle
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